Sensitive detection of malaria infection by third harmonic generation imaging
Sensitive detection of malaria infection by third harmonic generation imaging
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DOI：
10.1529/biophysj.107.125443
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发表时间：
2008-02-15
影响因子：
3.4
通讯作者：
Wiseman, Paul W.
中科院分区：
文献类型：
--
作者：
Belisle, Jonathan M.;Costantino, Santiago;Wiseman, Paul W.
疟疾仍然是全世界的一个主要健康问题，在疟疾流行的国家，每年报告有3.5亿至5亿病例。在这项研究中，我们报告了一种新的和高灵敏度的基于光学的检测疟疾感染的血细胞的疟原虫色素，自然沉积在其生命周期中的三次谐波产生（THG）成像。来自疟原虫色素的THG信号比我们在任何细胞类型中观察到的都要大，信噪比达到1000：1。该方法允许快速和稳健地检测血细胞的早期感染。巨大的非线性响应的内在寄生虫的副产物色素表明，自动光学检测THG可用于灵敏和快速筛选血液样品中的寄生虫感染。
Malaria remains a major health concern worldwide, with 350-500 million cases reported annually in endemic countries. In this study, we report a novel and highly sensitive optical-based detection of malaria-infected blood cells by third harmonic generation (THG) imaging of hemozoin pigment that is naturally deposited by the parasite during its lifecycle. The THG signal from the hemozoin was greater than we have observed in any cell type with signal/noise ratios that reach 1000:1. This method allows a rapid and robust detection of early stage infections of blood cells. The immense nonlinear response of the intrinsic parasitic by-product pigments suggests that automated optical detection by THG could be used for sensitive and rapid screening of parasite infection in blood samples.