Position estimation of fluorescent probes in a confocal microscope
Position estimation of fluorescent probes in a confocal microscope
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共焦显微镜中荧光探针的位置估计
DOI：
10.1109/cdc.2007.4434907
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发表时间：
2007
期刊：
影响因子：
--
通讯作者：
S. Andersson
中科院分区：
文献类型：
--
作者：
S. Andersson
共聚焦显微镜中孤立荧光探针的荧光强度测量可以看作是该探针位置的范围测量。我们提出了一种解析算法，用于从平面上不同位置的测量集合中确定其位置。这个算法，fluoroBancroft，是受到GPS中的Bancroft算法的启发。我们通过仿真研究了该算法的性能，并将其与拟合数据到高斯分布的标准技术进行了比较。我们的结果表明，当只使用少量测量时，fluoroBancroft算法比拟合程序更准确。此外，就计算时间而言，该技术通常要快两个数量级。这些初步结果表明，fluoroBancroft算法有望用于实时闭环控制器，以跟踪单个荧光探针的运动。
Measurements of fluorescence intensity from an isolated fluorescing probe in a confocal microscope can be viewed as range-only measurements of the position of that probe. We present an analytical algorithm for determining its position from a collection of measurements taken at different locations in the plane. This algorithm, fluoroBancroft, is inspired by the Bancroft algorithm in GPS. We investigate the performance of the algorithm through simulation and compare it to the standard technique of fitting the data to a Gaussian profile. Our results indicate that the fluoroBancroft algorithm is more accurate than the fitting procedure when only a few measurements are used. Moreover the technique is typically two orders of magnitude faster in terms of computation time. These preliminary results indicate the fluoroBancroft algorithm holds promise for use in a real-time closed-loop controller to track the motion of single fluorescent probes.
影响因子：
3.4
作者：
Thompson, RE;Larson, DR;Webb, WW
通讯作者：
Webb, WW
影响因子：
--
作者：
MCCUTCHEN, CW
通讯作者：
MCCUTCHEN, CW
影响因子：
3.4
作者：
Cheezum, MK;Walker, WF;Guilford, WH
通讯作者：
Guilford, WH