Characterization of four rice mutants with alterations in the defence response pathway
Characterization of four rice mutants with alterations in the defence response pathway
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DOI：
10.1111/1468-0025.00206-i1
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发表时间：
2005-01-01
影响因子：
4.9
通讯作者：
Ronald, PC
中科院分区：
文献类型：
--
作者：
Campbell, MA;Ronald, PC
利用水稻稻瘟病的致病因子Magnaporthe grisea对一个快中子诱变的水稻幼苗群体进行筛选，以确定防御反应发生改变的突变体。3个突变系ebr1、ebr2和ebr3对稻瘟病病菌的抗性增强。ebr1和ebr3也增强了对细菌病原体米黄单胞菌pv的抗性。oryzae (Xoo语)。ebr3在接种稻瘟杆菌后产生病变模拟（LM）表型，并且表型也可由环境条件的变化诱导。第四个突变系ncr1（水稻坏死）在所有测试条件下都具有LM表型，并且对稻瘟病杆菌或Xoo缺乏增强的抗性。利用突变系ebr3和ncr1进行的互补测试表明，ebr3和ncr1位点是非等位基因和隐性的。与野生型C039相比，ebr1和ebr2的水稻致病相关基因PBZ1和PR1的表达没有变化。ebr3仅在LM表型的组织中表达PBZ1和PR1。ncr1在LM表型组织中强烈表达PBZ1，而PR1在LM表型组织中的表达与野生型C039相似。
A fast-neutron mutagenized population of rice seedlings was screened with Magnaporthe grisea, the causal agent of rice blast disease, to identify mutants with alterations in the defence response. Three mutant lines, ebr1, ebr2 and ebr3 (enhanced blast resistance) were identified that display enhanced resistance to M. grisea. ebr1 and ebr3 also confer enhanced resistance to the bacterial pathogen Xanthomonas oryzae pv. oryzae (Xoo). ebr3 develops a lesion mimic (LM) phenotype upon inoculation with M. grisea, and the phenotype is also induced by a shift in environmental conditions. The fourth mutant line, ncr1 (necrosis in rice), has an LM phenotype under all conditions tested and lacks enhanced resistance to either M. grisea or Xoo. Complementation testing using the mutant lines ebr3 and ncr1 indicates that the ebr3 and ncr1 loci are nonallelic and recessive. ebr1 and ebr2 display no alterations in expression of the rice pathogenesis-related (PR) genes PBZ1 and PR1, compared to wild-type C039. ebr3 has an elevated expression of PBZ1 and PR1 only in tissue displaying the LM phenotype. ncr1 strongly expresses PBZ1 in tissue displaying the LM phenotype, whereas PR1 expression in this tissue is similar to wild-type C039.