Henriciella marina gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from the East Sea
Henriciella marina gen. nov., sp. nov., a novel member of the family Hyphomonadaceae isolated from the East Sea
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DOI：
10.1007/s12275-008-0290-0
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发表时间：
2009-05
期刊：
影响因子：
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通讯作者：
Zhe-Xue Quail;Dan-Ning Zeng;Yi-ping Xiao;S. Roh;Y. Nam;Ho-Won Chang;J. Yoon;H. Oh;J. Bae
中科院分区：
文献类型：
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作者：
Zhe-Xue Quail;Dan-Ning Zeng;Yi-ping Xiao;S. Roh;Y. Nam;Ho-Won Chang;J. Yoon;H. Oh;J. Bae
从韩国东海分离到一株细菌，命名为Iso4T，并对其进行了多相分类研究，包括表型和化学分类特征以及16S rRNA基因序列分析。该菌株的细胞革兰氏阴性，能动，无芽，无柄，严格需氧。菌株Iso4T在20℃、1∼、2%(w/v)氯化钠、pH 6.9、∼7.6条件下生长最好。细胞内主要脂肪酸为C18：1ω7c(53.5%)、C17：1ω5c(11.7%)、C17：1ω6c(8.1%)、C16：0(7.8%)、C17：0(4.8%)、C15：0(2.9%)和C16：1ω5c(2.2%)。菌株Iso4T的DNA G+C含量为56.2mol%。基于16S rRNA基因序列的系统发育分析表明，菌株Iso4T在水单胞菌科中形成了一个单系分支，由较高的自助值支持，与该家族中的海洋细菌属Hyphomonas(92∼94%)亲缘关系最近。表型、基因分型和化学分类的证据也表明，菌株Iso4Tree是丝状单胞菌科的一个新属和新种，命名为Henriciellagen。Nov.，sp.11月是提议的。模式菌株为Iso4T(=KCTC 12513T=DSM 19595T=JCM 15116T)。
A bacterial strain, designated Iso4T, was isolated from the East Sea of Korea and was subjected to a poly-phasic taxonomy study including phenotypic and chemotaxonomic characteristics as well as 16S rRNA gene sequence analysis. Cells of the strain were Gram-negative, motile, non-budding, non-stalked, and strictly aerobic. Strain Iso4Tgrew optimally at 20°C in the presence of 1∼2% (w/v) NaCl and at pH 6.9∼7.6. The major respiratory quinone was Q-10 and the major cellular fatty acids were C18:1ω7c (53.5%), C17:1ω5c (11.7%), C17:1ω6c (8.1%), C16:0(7.8%), C17:0(4.8%), C15:0(2.9%), and C16:1ω5c (2.2%). The DNA G+C content of strain Iso4Twas 56.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain Iso4Tformed a monophyletic clade in the familyHyphomonadaceae, supported by high bootstrap value and was most closely related to the genusHyphomonas(92∼94%), a member of marine bacteria in the family. The phenotypic, genotypic, and chemotaxonomic evidences also suggest strain Iso4Trepresents a novel genus and species in the familyHyphomonadaceae, for which the nameHenriciellagen. nov., sp. nov. is proposed. The type strain is Iso4T(=KCTC 12513T=DSM 19595T=JCM 15116T).