Fabrication of Microbial Biofilm Arrays by Geometric Control of Cell Adhesion
Fabrication of Microbial Biofilm Arrays by Geometric Control of Cell Adhesion
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DOI：
10.1021/la803985a
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发表时间：
2009-04-21
期刊：
影响因子：
3.9
通讯作者：
Weibel, Douglas B.
中科院分区：
文献类型：
--
作者：
Eun, Ye-Jin;Weibel, Douglas B.
本文提出了一种利用薄聚合物支架在各种不同基底上形成微生物生物膜阵列的技术。支架作为“支架”发挥作用，其提供对表面上的细胞粘附的几何控制并将生物膜生长限制在基底的特定区域。我们展示了具有特征的生物膜阵列的制造（例如，单个生物膜）直径小至50 μ m，具有可再现的生理特性。使用该技术可以产生一系列微生物的生物膜阵列，包括：铜绿假单胞菌，B。subtilis、S. epidermidis、V.fischeri、E. coli和C.白色念珠菌这种方法提供了一种简单的，用户可配置的，并且相对便宜的方法，用于在静态和流动条件下生长生物膜。本文中描述的方法使得有可能研究的化学，物理和环境因素，影响生物膜的发展，在统计相关和可重复的格式。
This paper presents a technique for patterning arrays of microbial biofilms on a wide range of different substrates using thin polymer stencils. The stencils function as "scaffolds" that provide geometric control over cell adhesion on surfaces and confine biofilm growth to specific regions of a substrate. We demonstrate the fabrication of biofilm arrays with features (e.g., individual biofilms) as small as 50 mu m in diameter with physiological characteristics that are reproducible. Biofilm arrays of a range of microorganisms can be produced using this technique, including: P. aeruginosa, B. subtilis, S. epidermidis, V. fischeri, E. coli, and C. albicans. This approach provides a simple, user-configurable, and relatively inexpensive method for growing biofilms in both static and now conditions. The method described in this paper makes it possible to study the chemical, physical, and environmental factors that affect biofilm development in a statistically relevant and reproducible format.