Evaluation of the replication and pathogenicity of a variant avian paramyxovirus serotype 6 in mice
Evaluation of the replication and pathogenicity of a variant avian paramyxovirus serotype 6 in mice
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DOI：
10.1007/s00705-017-3459-1
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发表时间：
2017-07
影响因子：
2.7
通讯作者：
V. N. Bui;D. Q. Trinh;Lary N. B. Abao;Y. Ozeki;J. Runstadler;Kikuyasu Nakamura;H. Ogawa;K. Imai
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文献类型：
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作者：
V. N. Bui;D. Q. Trinh;Lary N. B. Abao;Y. Ozeki;J. Runstadler;Kikuyasu Nakamura;H. Ogawa;K. Imai
禽类副粘病毒(APMV)作为疫苗载体的潜在用途已被评估，这引发了研究努力，从而更好地了解APMV的复制和致病性。然而，在APMV血清型中，存在着显著的遗传多样性，并且哺乳动物中变异株的感染性尚未被研究。我们利用小鼠模型评价了APMV-6变异株(APMV-6/Red-Neck Stint/Japan/8KS0813/2008)与APMV-6原型株(APMV-6/鸭子/香港/18/199/1977)的致病性。虽然这两种病毒在基因和抗原性上都有很大的不同，但我们发现变异株和原型株类似地可以在感染的小鼠的呼吸道组织中复制并引发呼吸道疾病，有时会导致小鼠死亡。这两种病毒都能诱导体液免疫反应，但用血凝抑制试验却很难识别。
Avian paramyxoviruses (APMVs) have been evaluated for their potential use as vaccine vectors, sparking research efforts leading to a better understanding of APMVs’ replication and pathogenicity. However, within APMV serotypes, significant genetic diversity exists, and the infectivity of variant strains in mammals has not been studied. We utilized a mouse model to evaluate the pathogenicity of a variant strain of APMV-6 (APMV-6/red-necked stint/Japan/8KS0813/2008) in comparison with the prototype APMV-6 strain (APMV–6/duck/Hong Kong/18/199/1977). Although the two viruses differ substantially, both genetically and antigenically, we found that the variant and prototype strains could similarly replicate in respiratory tissues of infected mice and induce respiratory disease, sometimes resulting in death of the mice. Both viruses induced a humoral immune response that could be clearly detected by ELISA but which was poorly recognized by the hemagglutination inhibition test.