Selective expansion of target cells using the Enrich TROVO platform.
Selective expansion of target cells using the Enrich TROVO platform.
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DOI：
10.2144/btn-2023-0038
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发表时间：
2023-08
期刊：
影响因子：
2.7
通讯作者：
中科院分区：
文献类型：
--
作者：
从不同细胞群中富集靶细胞克隆对于许多生命科学应用至关重要。我们开发了一种新方法，可以使用 Enrich TroVo 系统（Enrich Biosystems Inc.，康涅狄格州，美国）从更大的异质群体中快速有效地纯化特定克隆细胞群。该系统利用微加工和光学技术，利用小型水凝胶孔分离所需的细胞群，并利用创新的修补技术选择性地消除不需要的细胞。该方法允许所需细胞的分离和生长，同时对其活力和增殖的影响最小。我们使用两种模型细胞成功分离并扩增了所需细胞的克隆细胞群。与荧光激活细胞分选相比，Enrich TroVo 系统为分离敏感的贴壁细胞（即患者来源的细胞）提供了一种有前景的替代方案。
Enriching target cell clones from diverse cell populations is vital for many life science applications. We have developed a novel method to rapidly and efficiently purify specific clonal cell populations from a larger, heterogeneous group using the Enrich TroVo system (Enrich Biosystems Inc., CT, USA). This system takes advantage of microfabrication and optical technologies by utilizing small hydrogel wells to separate desired cell populations and an innovative patching technique to selectively eliminate undesired cells. This method allows the isolation and growth of desired cells with minimal impact on their viability and proliferation. We successfully isolated and expanded clonal cell populations of desired cells using two model cells. Compared with fluorescence-activated cell sorting, Enrich TroVo system offers a promising alternative for isolating of sensitive, adherent cells, that is, patient-derived cells.
影响因子：
4.6
作者：
Romita L;Thompson S;Hwang DK
通讯作者：
Hwang DK