Comparative transcriptome analysis of Yersinia pestis in response to hyperosmotic and high-salinity stress
Comparative transcriptome analysis of Yersinia pestis in response to hyperosmotic and high-salinity stress
复制标题
鼠疫耶尔森菌响应高渗和高盐胁迫的比较转录组分析
DOI：
10.1016/j.resmic.2004.10.004
复制
发表时间：
2005-04-01
影响因子：
2.6
通讯作者：
Yang, RF
中科院分区：
文献类型：
--
作者：
Han, YP;Zhou, DS;Yang, RF
DNA 微阵列被用作研究鼠疫耶尔森氏菌对高渗和高盐胁迫的全基因组转录反应的工具。高渗胁迫特别上调负责 ABC 型运输和某些多糖细胞质积累的基因，而高盐胁迫则诱导编码分区蛋白和几种全局转录调节因子的基因转录。两种胁迫下转录均得到增强的基因包括编码渗透保护转运系统和一组毒力决定子的基因。两种应激下调的基因数量远低于上调基因的数量，表明这两种应激总体上都不会严重抑制细胞过程。许多差异调节基因仍然存在，但其功能仍未知。鼠疫耶尔森氏菌将高盐度和高渗胁迫视为不同种类的环境刺激，并且不同的机制能够适应这两种胁迫，尽管鼠疫耶尔森氏菌仍然执行共同的机制来适应这两种类型的胁迫。 (c) 2004 年爱思唯尔 SAS。版权所有。
DNA microarray was used as a tool to investigate genome-wide transcriptional responses of Yersinia pestis to hyperosmotic and high-salinity stress. Hyperosmotic stress specifically upregulated genes responsible for ABC-type transport and the cytoplasmic accumulation of certain polysaccharides, while high-salinity stress induced the transcription of genes encoding partition proteins and several global transcriptional regulators. Genes whose transcription was enhanced by both kinds of stress comprised those encoding osmoprotectant transport systems and a set of virulence determinants. The number of genes downregulated by the two kinds of stress was much lower than that of upregulated genes, suggesting that neither kind of stress severely depresses cellular processes in general. Many differentially regulated genes still exist whose functions remain unknown. Y. pestis recognized high-salinity and hyperosmotic stress as different kinds of environmental stimuli, and different mechanisms enabled acclimation to these two kinds of stress, although Y. pestis still executed common mechanisms to accommodate both types of stress. (c) 2004 Elsevier SAS. All rights reserved.