LAMINA PROPRIA T-CELL SUBSETS IN THE SMALL AND LARGE-INTESTINE OF EUTHYMIC AND ATHYMIC MICE
LAMINA PROPRIA T-CELL SUBSETS IN THE SMALL AND LARGE-INTESTINE OF EUTHYMIC AND ATHYMIC MICE
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DOI：
10.1111/j.1365-3083.1995.tb03645.x
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发表时间：
1995-08-01
影响因子：
3.7
通讯作者：
REIMANN, J
中科院分区：
文献类型：
--
作者：
BOLL, G;REIMANN, J
我们研究了来自正常胸腺（BALB/c、C.B-17、C57 BL/6）和无胸腺（C57 BL/6 nu/nu; BNX bg/bg nu/nu xid/xid）小鼠的小肠（空肠/回肠）和大肠（结肠）的固有层T细胞。正常小鼠小肠或大肠固有层淋巴细胞（LPL）中CD 3（+）T细胞约占40%，无胸腺小鼠小肠或大肠固有层淋巴细胞中CD 3（+）T细胞占20-30%。在小肠固有层T细胞群中，正常胸腺小鼠中85%为α β谱系，但无胸腺小鼠中仅40%为α β谱系。因此，在胸腺外起源的肠固有层T细胞中，γ-δ谱系的T细胞比α-β谱系的T细胞更频繁。在正常小鼠小肠和大肠中，CD 4（+）T细胞占固有层T细胞的40%，在裸鼠肠道固有层中占20-30%。在正常胸腺小鼠中，小肠固有层中40%的T细胞和结肠固有层中30%的T细胞是CD 8（+）。在无胸腺小鼠的肠固有层T细胞群中，CD 8（+）T细胞群扩增。正常胸腺和无胸腺小鼠小肠和大肠固有层中的大多数（60-70%）CD 8（+）T细胞表达CD 8辅助受体的同型二聚体CD 8 α（+）β（-）形式。正常胸腺和无胸腺小鼠小肠和大肠固有层中所有CD 3（+）T细胞中有15-20%是“双阴性”CD 4（-）CD 8（-）。正常胸腺小鼠结肠固有层（但小肠固有层中不存在）中的大部分TCR α β（+）T细胞表达CD 2和CD 28共刺激分子、粘附分子LECAM-1（CD 62 L），并可通过CD 3连接在体外激活。这些数据揭示了小鼠固有层T细胞的表面表型和功能表型的相当大的异质性。
We investigated lamina propria T cells from the small intestine (jejunum/ileum) and the large intestine (colon) of euthymic (BALB/c, C.B-17, C57BL/6) and athymic (C57BL/6 nu/nu; BNX bg/bg nu/nu xid/xid) mice. CD3(+) T cells represented about 40% of the lamina propria lymphocytes (LPL) from the small or the large intestine of euthymic mice, and 20-30% of the LPL populations from the small or large intestine of athymic mice. In the lamina propria T cell population of the small intestine, 85% were of the alpha beta lineage in euthymic mice, but only 40% were of the alpha beta lineage in athymic mice. T cells of the gamma delta lineage were thus more frequent than T cells of the alpha beta lineage in the intestinal lamina propria T cells of extrathymic origin. CD4(+) T cells represented 40% of the lamina propria T cells in the small as well as in the large intestine of euthymic mice, and 20-30% of the T cells in the lamina propria of the nude mouse gut. In euthymic mice, 40% of the T cells in the small intestine lamina propria, and 30% of the T cells in the colonic lamina propria were CD8(+). In intestinal lamina propria T cell populations of athymic mice, the CD8(+) T cell population was expanded. Most (60-70%) CD8(+) T cells in the lamina propria of the small and the large intestine of euthymic and athymic mice expressed the homodimeric CD8 alpha(+)beta(-) form of the CD8 coreceptor. A fraction of 15-20% of all CD3(+) T cells in the lamina propria of the small and the large intestine of euthymic and athymic mice were 'double negative' CD4(-)CD8(-). A large fraction of the TCR alpha beta(+) T cells in the colonic lamina propria (but not in the small intestine lamina propria) of euthymic mice expressed the CD2 and the CD28 costimulator molecules, the adhesion molecule LECAM-1 (CD62 L), and could be activated in vitro by CD3 ligation. These data reveal a considerable heterogeneity in the surface phenotype and the functional phenotype of murine lamina propria T cells.