Large-scale transcriptional activity in chromosomes 21 and 22
Large-scale transcriptional activity in chromosomes 21 and 22
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DOI：
10.1126/science.1068597
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发表时间：
2002-05-03
期刊：
影响因子：
56.9
通讯作者：
Gingeras, TR
中科院分区：
文献类型：
--
作者：
Kapranov, P;Cawley, SE;Gingeras, TR
人类21号和22号染色体的序列表明，大约有770个已被充分表征和预测的基因。在这项研究中，利用从11个人类细胞系中获得的胞质聚腺苷化RNA构建了鉴定这些染色体上RNA转录活性区域的经验衍生图谱。沿着这些染色体平均每隔35个碱基对的探针组成的寡核苷酸阵列被使用。当与这些染色体可用的序列注释进行比较时，可以注意到，与预测和表征的外显子相比，转录的基因组序列要多出一个数量级。
The sequences of the human chromosomes 21 and 22 indicate that there are approximately 770 well-characterized and predicted genes. In this study, empirically derived maps identifying active areas of RNA transcription on these chromosomes have been constructed with the use of cytosolic polyadenylated RNA obtained from 11 human cell lines. Oligonucleotide arrays containing probes spaced on average every 35 base pairs along these chromosomes were used. When compared with the sequence annotations available for these chromosomes, it is noted that as much as an order of magnitude more of the genomic sequence is transcribed than accounted for by the predicted and characterized exons.