Synthesis and activity of NH2- and COOH-terminal elastase recognition sequences on cotton
Synthesis and activity of NH2- and COOH-terminal elastase recognition sequences on cotton
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DOI：
10.1034/j.1399-3011.1999.00134.x
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发表时间：
1999-12-01
期刊：
影响因子：
--
通讯作者：
Goheen, SC
中科院分区：
文献类型：
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作者：
Edwards, JV;Batiste, SL;Goheen, SC
将弹性蛋白酶肽识别序列应用于伤口敷料纤维是抑制慢性伤口中高水平破坏性弹性蛋白酶的可能途径。为此，我们在棉纤维素和羧甲基化纤维素棉（CMC）上合成了弹性酶识别序列Val-Pro-Val，并制备了这些序列的色谱柱来检测弹性酶保留。在棉基纤维素纤维上按顺序合成了三肽甲酯。甘氨酸作为识别序列与棉花纤维素的连接物。用纤维素酶对棉花纤维素进行预处理，提高了甘氨酸的取代水平。将肽纤维素偶联物作为色谱固定相来评估弹性蛋白酶的保留。Val-Pro-Val-OMe序列氨基末端固定在羧甲基化棉花上，首次应用于色谱柱时，弹性蛋白酶保留率高达58%。随后显示出较高的重复记忆。同样用Val-Pro-Val-Gly纤维素修饰的棉纱布与未处理的纱布进行比较，以降低缓冲盐水中的弹性酶活性。用Val-Pro-Val-Gly纤维素棉纱布处理的弹性酶溶液显示弹性酶活性降低。本研究展示了弹性蛋白酶识别序列作为弹性蛋白酶附着在棉纤维上的隔离剂的使用，并为慢性伤口敷料纤维中肽纤维素类似物的设计提供了模型。
The application of peptide recognition sequences of elastase to fibers of wound dressings is a possible route to inhibiting high levels of destructive elastase in the chronic wound. For this reason we have synthesized the elastase recognition sequence Val-Pro-Val on both cotton cellulose, and carboxymethylated cellulose cotton (CMC) and prepared chromatography columns of these to examine elastase retention. The tripepride was synthesized on cotton-based cellulose fibers both in sequence and as a tripeptide methyl ester. Glycine war employed as a linker of the recognition sequence to the cotton cellulose. Pretreatment of cotton cellulose with cellulase improved the substitution level of glycine. The peptidocellulose conjugates were employed asa chromatographic stationary phase to assess elastase retention. The sequence Val-Pro-Val-OMe was amino-terminally anchored to carboxymethylated cotton and demonstrated retention of up to 58% of elastase when first applied to the column. Higher repetitive retention was demonstrated subsequently. Cotton gauze similarly modified with Val-Pro-Val-Gly cellulose was compared with untreated gauze for reduction of elastase activity in buffered saline. Solutions of elastase that were treated with Val-Pro-Val-Gly cellulose cotton gauze, demonstrated reduced elastase activity. This study demonstrates the use of elastase recognition sequences as sequestering agents of elastase when attached to cotton fibers and constitutes a model far the design of peptidocellulose analogs in dressing fibers for chronic wounds.