TISSUE DISTRIBUTION OF MICROSOMAL CYTOCHROME-P-450 MONOOXYGENASES AND THEIR INDUCIBILITY BY PHENOBARBITAL IN THE INSECTICIDE RESISTANT LPR STRAIN OF HOUSE-FLY, MUSCA-DOMESTICA L
TISSUE DISTRIBUTION OF MICROSOMAL CYTOCHROME-P-450 MONOOXYGENASES AND THEIR INDUCIBILITY BY PHENOBARBITAL IN THE INSECTICIDE RESISTANT LPR STRAIN OF HOUSE-FLY, MUSCA-DOMESTICA L
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DOI：
10.1016/0965-1748(93)90047-v
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发表时间：
1993-09-01
影响因子：
3.8
通讯作者：
LEE, SST
中科院分区：
文献类型：
--
作者：
SCOTT, JG;LEE, SST
抗性品系家蝇近端肠(PI)、马氏管(MT)和脂肪体(Fb)细胞色素P-450s、细胞色素b5、P-450还原酶和4种P-450单加氧酶活性丰富。与杀虫剂敏感品系相比，LPR果蝇对杀虫剂的抗性与几乎所有这些组织中P-450单加氧酶活性的增加有关。细胞色素P-450lpr存在于所有组织中，并且在LPR中的水平高于敏感果蝇，这表明P-450lpr在单加氧酶介导的杀虫剂抗性中的作用并不是由于它在单个组织中的水平较高。然而，由于脂肪小体相对较大，且P-450lpr在组织中含量丰富，因此脂肪体可能在抵抗中起主要作用。总细胞色素P-450s、细胞色素P-450lpr和P-450单加氧酶活性一般对苯巴比妥(PB)诱导的LPR无效。这与之前在易感家蝇中的报告形成对比，该报告表明P-450单加氧酶是由PB诱导的。总P-450、细胞色素P-450lpr和单加氧酶活性在家蝇的几种组织中呈结构性高表达。
Cytochrome P-450s, cytochrome b5, P-450 reductase, and four P-450 monooxygenase activities were abundant in proximal intestine (PI), Malpighian tubules (MT), and fat bodies (FB) in the insecticide resistant (LPR) strain of house fly. Insecticide resistance in LPR flies was associated with increases in P-450 monooxygenase activities in nearly all of these tissues relative to an insecticide susceptible strain. Cytochrome P-450lpr was present in all tissues and at higher levels in LPR than in susceptible flies suggesting that the role of P-450lpr in monooxygenase-mediated insecticide resistance is not due to its higher levels in a single tissue. However, it appears that fat bodies may have a major role in resistance due to their relatively large size and the abundance of P-450lpr in this tissue.Total cytochrome P-450s, cytochrome P-450lpr and P-450 monooxygenase activities were generally refractory to phenobarbital (PB) induction in LPR. This contrasts with a previous report in susceptible house flies showing that P-450 monooxygenases were induced by PB. It appears that total P-450s, cytochrome P-450lpr and monooxygenase activities were expressed at constitutive high levels in several tissues of LPR house flies.