Dimethylarsinous Acid Disturbs Cytokinesis
Dimethylarsinous Acid Disturbs Cytokinesis
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二甲基胂酸干扰细胞分裂
DOI：
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发表时间：
2009
影响因子：
2.7
通讯作者：
G. Endo
中科院分区：
文献类型：
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作者：
M. Kitamura;K. Kuroda;G. Endo
二甲基larsinous酸（DMAIII）诱导V79细胞四倍体和多核。当DMAIII浓度为1.25 μM时，四倍体和多核细胞的产量最高，当DMAIII浓度为2.5 μM时，有丝分裂指数最高。5 μM时未见有丝分裂。我们观察了DMAIII处理的V79细胞，并用罗丹明-phalloidin显像。DMAIII处理和罗丹明-phalloidin显像均可观察到分裂细胞肌动蛋白定位异常。这些发现强烈表明，DMAIII不仅抑制正常有丝分裂纺锤体的形成，而且抑制细胞分裂并诱导多核细胞的形成。
Dimethylarsinous acid (DMAIII) induced tetraploidy and multinucleation in V79 cells. While the highest yield of tetraploids and multinucleated cells was at 1.25 μM of DMAIII, the mitotic index was highest at DMAIII 2.5 μM due to mitotic arrest. Mitosis was not observed at 5 μM. We observed V79 cells treated with DMAIII and visualized with rhodamine-phalloidin. Abnormal actin location was observed in the dividing cells which were treated with DMAIII and visualized with rhodamine-phalloidin. These findings suggested strongly that DMAIII inhibits not only formation of the normal mitotic spindle but cytokinesis and induces the formation of multinuclear cells.