MORPHOLOGICAL FINDINGS IN LONG-TERM PANCREATIC TISSUE-TRANSPLANTS IN THE ANTERIOR EYE CHAMBER OF RATS
MORPHOLOGICAL FINDINGS IN LONG-TERM PANCREATIC TISSUE-TRANSPLANTS IN THE ANTERIOR EYE CHAMBER OF RATS
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DOI：
10.1097/00006676-199005000-00009
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发表时间：
1990-05-01
期刊：
影响因子：
2.9
通讯作者：
DONATH, T
中科院分区：
文献类型：
--
作者：
ADEGHATE, E;DONATH, T
采用光镜、电镜、乙酰胆碱酯酶和免疫组织化学方法研究移植到同源动物前眼房74、77、180和534天的21天龄胚胎和出生后2天大鼠胰腺组织片段的形态变化。胰腺腺泡细胞退化，在移植的这个阶段没有观察到。导管系统增殖并部分分化为内分泌细胞，随后也形成许多新的朗格汉斯岛。发现β-、α-、δ-和胰多肽细胞的结构甚至在移植后1.5年仍保持完整。除此之外，胰岛内这些细胞的组织与正常胰腺组织相似。移植的组织碎片具有良好的血管化，并且还受到血清素能细胞和乙酰胆碱酯酶阳性神经元的神经支配。结论是，除腺泡成分外的胰腺组织碎片可以在同源大鼠的前眼房中以完整的结构生长和存活长达1.5年。
Morphological changes in 21-day-old embryonic and 2-day-old postnatal rat pancreatic tissue fragments transplanted into the anterior eye chamber of homologous animals for 74, 77, 180, and 534 days were investigated using light and electron microscopic, acetylcholinesterase enzyme, and immunohistochemical methods. The pancreatic acinar cells degenerated and were not observed at this stage of transplantation. The ductal system proliferated and partly differentiated into endocrine cells that subsequently formed many new islets of Langerhans as well. The structures of .beta.-, .alpha.-, .delta.-, and pancreatic polypeptide cells were found to be intact even 1.5 years after transplantation. In addition to this, the organization of these cells inside the islets is similar to that of normal pancreatic tissue. The transplanted tissue fragments were well vascularized with blood vessels and innervated also by serotonergic cells and acetylcholinesterase-positive neurons. It is concluded that pancreatic tissue fragments, with the exception of the acinar component, can grow and survive with intact structure in the anterior eye chamber of homologous rats for up to 1.5 years.