Probiotic-fermented purple sweet potato yogurt activates compensatory IGF-IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats
Probiotic-fermented purple sweet potato yogurt activates compensatory IGF-IR/PI3K/Akt survival pathways and attenuates cardiac apoptosis in the hearts of spontaneously hypertensive rats
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DOI：
10.3892/ijmm.2013.1524
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发表时间：
2013-12-01
影响因子：
5.4
通讯作者：
Tsai, Cheng-Chih
中科院分区：
文献类型：
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作者：
Lin, Pei-Pei;Hsieh, You-Miin;Tsai, Cheng-Chih
细胞凋亡被认为是心脏病患者不良结局的预测因子。本研究旨在探讨高氨基丁酸(GABA)含量的益生菌发酵紫薯酸奶(PSPY)对自发性高血压大鼠(SHR)心脏细胞凋亡的影响。大鼠灌胃给予两种不同浓度的PSPY(10%和100%)或卡托普利15.6 mg/kg，体重(BW)/d。对照组给予蒸馏水。DAPI和TUNEL染色检测细胞凋亡数。SHR-PSPY(10%和100%)组心肌细胞TUNEL阳性细胞数明显减少。此外，免疫印迹分析还检测了依赖Fas受体和线粒体的凋亡通路的关键成分的水平。结果显示，SHR-Captopril组、10%PSPY组和100%PSPY组Fas受体和线粒体依赖的细胞凋亡途径的关键成分的水平显著降低。此外，SHR对照组的SHR心脏中磷酸化的胰岛素样生长因子-I受体(p-IGF-IR)水平增加，但下游信号成分的水平没有恢复。此外，SHR-10组和100%PSPY组大鼠左室心肌中IGF-IR依赖的代偿性生存通路(p-PI3K和p-Akt)的含量均显著升高。因此，口服PSPY可能通过激活IGF-IR依赖的生存信号通路来减轻SHR心肌细胞的凋亡。
Apoptosis is recognized as a predictor of adverse outcomes in subjects with cardiac diseases. The aim of this study was to explore the effects of probiotic-fermented purple sweet potato yogurt (PSPY) with high -aminobutyric acid (GABA) content on cardiac apoptosis in spontaneously hypertensive rat (SHR) hearts. The rats were orally adminsitered with 2 different concentrations of PSPY (10 and 100%) or captopril, 15.6 mg/kg, body weight (BW)/day. The control group was administered distilled water. DAPI and TUNEL staining were used to detect the numbers of apoptotic cells. A decrease in the number of TUNEL-positive cardiac myocytes was observed in the SHR-PSPY (10 and 100%) groups. In addition, the levels of key components of the Fas receptor- and mitochondrial-dependent apoptotic pathways were determined by western blot analysis. The results revealed that the levels of the key components of the Fas receptor- and mitochondrial-dependent apoptotic pathway were significantly decreased in the SHR-captopril, and 10 and 100% PSPY groups. Additionally, the levels of phosphorylated insulin-like growth factor-I receptor (p-IGF-IR) were increased in SHR hearts from the SHR-control group; however, no recovery in the levels of downstream signaling components was observed. In addition, the levels of components of the compensatory IGF-IR-dependent survival pathway (p-PI3K and p-Akt) were all highly enhanced in the left ventricles in the hearts form the SHR-10 and 100% PSPY groups. Therefore, the oral administration of PSPY may attenuate cardiomyocyte apoptosis in SHR hearts by activating IGF-IR-dependent survival signaling pathways.