AMINO TERMINUS OF THE YEAST GAL4 GENE-PRODUCT IS SUFFICIENT FOR NUCLEAR-LOCALIZATION
AMINO TERMINUS OF THE YEAST GAL4 GENE-PRODUCT IS SUFFICIENT FOR NUCLEAR-LOCALIZATION
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DOI：
10.1073/pnas.81.19.5951
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发表时间：
1984-01-01
期刊：
影响因子：
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通讯作者：
PTASHNE, M
中科院分区：
文献类型：
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作者：
SILVER, PA;KEEGAN, LP;PTASHNE, M
大肠杆菌的酵母[酿酒酵母]细胞内区隔化。研究了氨基端携带异源氨基酸序列的-半乳糖苷酶。在酵母正调节蛋白GAL4的氨基端，含有74个nh2末端氨基酸的嵌合体在细胞核中积累。GAL4基因产物可能通过与DNA结合介导阳性控制；核定位的信息存在于它的氨基末端。GAL4氨基末端的氨基酸序列与先前提出的负责核定位的共识序列不一致。度量。-半乳糖苷酶在含有非核嵌合蛋白的细胞中的活性比在含有特异性集中在细胞核的嵌合蛋白的细胞中的活性高10倍。
The intracellular compartmentalization in yeast [Saccharomyces cerevisiae] of Escherichia coli .beta.-galactosidase bearing heterologous amino acid sequences at its amino terminus were studied. Chimeras containing as few as 74 NH2-terminal amino acids of GAL4, a yeast positive regulatory protein, at the amino terminus accumulate in the cell nucleus. The GAL4 gene product may mediate positive control by binding to DNA; information for nuclear localization resides in its amino terminus. The amino acid sequence of the GAL4 amino terminus does not agree with the previously proposed consensus sequences responsible for nuclear localization. The .beta.-galactosidase activity in cells bearing the non-nuclear chimeric proteins is 10-fold greater than in cells bearing chimeric proteins that specifically concentrate in the nucleus.