Bromodeoxyuridine-labeled viral particles as a tool for visualization of the immediate-early events of human cytomegalovirus infection.
Bromodeoxyuridine-labeled viral particles as a tool for visualization of the immediate-early events of human cytomegalovirus infection.
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溴脱氧尿苷标记的病毒颗粒作为人类巨细胞病毒感染早期事件可视化的工具。
DOI：
10.1128/jvi.78.14.7818-7822.2004
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发表时间：
2004
影响因子：
5.4
通讯作者：
Fortunato,ElizabethA
中科院分区：
文献类型：
--
作者：
Rosenke,Kyle;Fortunato,ElizabethA
我们在这里描述了一个简单的方法标记的基因组的人巨细胞病毒，一个大的双链DNA病毒，溴脱氧尿苷（BrdU）。将标记的DNA掺入病毒颗粒中，然后收集在细胞上清液中。为了证明这种方法的通用性和有效性，标记的病毒粒子被用来研究通过间接免疫荧光显微镜病毒-宿主细胞相互作用的立即早期事件。我们希望这种新的方法将被证明是有用的结合，进入和病毒基因组沉积在不同的病毒系统的研究。
We describe here a simple method for labeling the genome of human cytomegalovirus, a large double-stranded DNA virus, with bromodeoxyuridine (BrdU). The labeled DNA was incorporated into viral particles, which were then collected in cell supernatant. To demonstrate the versatility and effectiveness of this method, labeled virions were used to study the immediate-early events of virus-host cell interaction via indirect immunofluorescence microscopy. It is our hope that this new methodology will prove useful in the study of binding, entry and viral genome deposition in diverse virus systems.