Competitive ligand-binding assay for measurement of sex hormone-binding globulin (SHBG).
Competitive ligand-binding assay for measurement of sex hormone-binding globulin (SHBG).
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用于测量性激素结合球蛋白 (SHBG) 的竞争性配体结合测定。
DOI：
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发表时间：
1973
影响因子：
5.8
通讯作者：
Inder J. Chopra
中科院分区：
文献类型：
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作者：
Dan Tulchinsky;Inder J. Chopra
摘要 基于在性激素结合球蛋白 (SHBG) 和固定量的兔抗 DHT 之间分配恒定量的放射性标记配体 3H-二氢睾酮 (DHT) 的竞争性配体结合测定 (CLBA) 已用于测量血清 SHBG 浓度。该方法简便、灵敏、精密、重复性好，能准确测量SHBG浓度差异。血清白蛋白和皮质类固醇结合球蛋白不干扰该测定。在人血清中 SHBG 浓度和未结合 E2 百分比之间观察到一致的负相关。新生男婴的平均（±sem）血清SHBG浓度为0.31±0.02 U/ml，与新生女婴的平均（±sem）血清SHBG浓度（0.29±0.02 U/ml）没有显着差异。青春期前男孩的平均值为 0.22 ± 0.03 U/ml，低于新生儿值，但高于男性的 0.12 ± 0.01 U/ml。 10 名肝硬化男性的 SHBG 水平与正常值没有差异，但 10 名甲状腺功能亢进男性的 SHBG 水平为 6-7 倍...
ABSTRACT A competitive ligand-binding assay (CLBA) based on partition of a constant amount of radiolabeled ligand, 3H-dihydrotestosterone (DHT) between sex hormone-binding globulin (SHBG) and a fixed amount of rabbit anti-DHT has been utilized to measure serum SHBG concentration. The method is convenient, sensitive, precise and reproducible and it accurately measures differences in SHBG concentration. Serum albumin and corticosteroid-binding globulin do not interfere in this assay. A consistent inverse correlation was observed between SHBG concentrations and the percent unbound E2 in human serum. The mean (±sem) serum SHBG concentration in newborn infant boys of 0.31 ± 0.02 U/ml was not significantly different from that in newborn infant girls (0.29 ± 0.02 U/ml). The mean value of 0.22 ± 0.03 U/ml in prepubertal boys was lower than newborn value but higher than 0.12 ± 0.01 U/ml observed in men. SHBG levels of 10 cirrhotic men were not different from normal values but that of 10 hyperthyroid men was 6–7-fo...