ISOLATION AND CHARACTERIZATION OF A NOVEL PROTEIN (X-ORF PRODUCT) FROM SIV AND HIV-2
ISOLATION AND CHARACTERIZATION OF A NOVEL PROTEIN (X-ORF PRODUCT) FROM SIV AND HIV-2
复制标题
DOI：
10.1126/science.3388031
复制
发表时间：
1988-07-08
期刊：
影响因子：
56.9
通讯作者：
OROSZLAN, S
中科院分区：
文献类型：
--
作者：
HENDERSON, LE;SOWDER, RC;OROSZLAN, S
从猴免疫缺陷病毒（SIVMne）中纯化了一种命名为p14的蛋白质，氨基酸序列分析表明，该蛋白质与SIVmac和人类免疫缺陷病毒2型（HIV-2）核苷酸序列中独特的开放阅读框（X-ORF）的预测翻译产物几乎相同。因此，X-ORF被证明是一个新的逆转录病毒基因。p14在SIVMne中的摩尔量相当于gag蛋白的摩尔量。这是第一个逆转录病毒含有大量不是gag、pro、pol或env基因产物的病毒蛋白的例子。SIV p14及其在HIV-2中的同源物可以作为核酸结合蛋白，因为纯化的p14在体外结合单链核酸。纯化蛋白的抗血清检测到SIVMne，SIVmac中的p14和HIV-2中的同源蛋白（16千道尔顿），但不与HIV-1反应。基于这种新蛋白的诊断程序将区分HIV-1和HIV-2。
A protein designated p14 was purified from a simian immunodeficiency virus (SIVMne) and was shown by amino acid sequence analysis to be nearly identical to the predicted translational product of a unique open reading frame (X-ORF) in the nucleotide sequences of SIVmac and human immunodeficiency virus type 2 (HIV-2). Thus the X-ORF is proven to be a new retroviral gene. The p14 is present in SIVMne in molar amounts equivalent to those of the gag proteins. This is the first example of a retrovirus that contains a substantial quantity of a viral protein that is not a product of the gag, pro, pol, or env genes. SIV p14 and its homolog in HIV-2 may function as nucleic acid binding proteins since purified p14 binds to single-stranded nucleic acids in vitro. Antisera to the purified protein detected p14 in SIVMne, SIVmac, and a homologous protein (16 kilodaltons) in HIV-2 but did not react with HIV-1. Diagnostic procedures based on this novel protein will distinguish between HIV-1 and HIV-2.