Electrostatically self-assembled biodegradable microparticles from pseudoproteins and polysaccharide: fabrication, characterization, and biological properties.
Electrostatically self-assembled biodegradable microparticles from pseudoproteins and polysaccharide: fabrication, characterization, and biological properties.
复制标题
由假蛋白和多糖静电自组装的可生物降解微粒：制造、表征和生物学特性。
DOI：
10.1021/bm5016255
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发表时间：
2015
影响因子：
6.2
通讯作者：
Chu,CC
中科院分区：
文献类型：
--
作者：
Potuck,AliciaN;Weed,BethL;Leifer,CynthiaA;Chu,CC
以新型阳离子不饱和精氨酸聚酯酰胺(UARG-PEA)和阴离子透明质酸(HA)为原料，采用静电自组装法制备了亚微米级的杂化微粒，并对其进行了紫外光交联以稳定其结构。对这些杂化微粒的大小、电荷、粘度、化学结构、形态和生物学特性进行了表征。根据阳离子UARG-PEA和阴离子HA的投料比不同，交联的微球形成直径为0.772~22.08μm的球形结构，而未交联的微球形成直径为2.49~15μm的花瓣状核心结构。研究发现，自组装微粒的形态结构对其生物学特性有着深刻的影响。当UARG-PEA与HA的投料比为1：1时，未交联微粒子在浓度达20μg/mL时对NIH3T3成纤维细胞无细胞毒性，而在浓度达10μg/mL时对成纤维细胞无细胞毒作用。UARG-PEA/HA杂化微粒表现出明显低于单纯透明质酸对照的巨噬细胞诱导的促炎反应(通过肿瘤坏死因子-α)，同时保留了HA有益的抗炎IL-10的产生。UARG-PEA/HA微粒还能刺激精氨酸酶活性的大小依赖性诱导。因此，将这两类生物材料在良好的无毒水环境中自组装，具有目前报道的UARG-PEA/HA杂化微粒的互补生物学特性，可能会提供一类新的生物材料，以改善整体组织微环境，促进伤口愈合。
Electrostatically self-assembling hybrid microparticles derived from novel cationic unsaturated arginine-based poly(ester amide) polymers (UArg-PEA) and anionic hyaluronic acid (HA) were fabricated into sub-micron-sized particles in aqueous medium with subsequent UV crosslinking treatment to stabilize the structure. These hybrid microparticles were characterized for size, charge, viscosity, chemical structure, morphology, and biological properties. Depending on the feed ratio of cationic UArg-PEA to anionic HA, the crosslinked microparticles formed spherical structures of 0.772–22.08 μm in diameter, whereas the uncrosslinked microparticles formed a core with an outer petal-like structure of 2.49–15 μm in diameter. It was discovered that the morphological structure of the self-assembled microparticles had a profound influence on their biological properties. At a 1:1 feed ratio of UArg-PEA to HA, the uncrosslinked microparticles showed no cytotoxicity toward NIH 3T3 fibroblasts at concentrations up to 20 μg/mL, and the crosslinked particles exhibited no cytotoxicity at concentrations up to 10 μg/mL. The UArg-PEA/HA hybrid microparticles exhibited a significantly lower macrophage-induced proinflammatory response (via TNF-α) than that from a pure hyaluronic acid control while retaining the beneficial anti-inflammatory IL-10 production by HA. The UArg-PEA/HA microparticles also stimulated size-dependent induction of arginase activity. Therefore, self-assembling these two types of biomaterials in a favorable nontoxic aqueous environment, having complementary biological properties like those of the currently reported UArg-PEA/HA hybrid microparticles, may provide a new class of biomaterials to improve the overall tissue microenvironment for promoting wound healing.