Carbachol stimulates a different phospholipid metabolism than nerve growth factor and basic fibroblast growth factor in PC12 cells.
Carbachol stimulates a different phospholipid metabolism than nerve growth factor and basic fibroblast growth factor in PC12 cells.
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在 PC12 细胞中，卡巴胆碱刺激的磷脂代谢与神经生长因子和碱性成纤维细胞生长因子不同。
DOI：
10.1091/mbc.2.5.383
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发表时间：
1991
期刊：
影响因子：
--
通讯作者：
Raben,DM
中科院分区：
文献类型：
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作者：
Pessin,MS;Altin,JG;Jarpe,M;Tansley,F;Bradshaw,RA;Raben,DM
我们检测了PC12细胞产生的1，2-二甘油酯(DGs)，并将它们与分化因子神经生长因子和碱性成纤维细胞生长因子反应产生的DGs进行了比较。虽然卡巴胆碱能刺激更多的肌醇磷酸盐的释放，但这三种激动剂产生的DGs水平相似。在这份报告中，我们分析了PC12 DGs对这三种激动剂的反应所产生的分子种类。此外，我们还分析了PC12磷脂的分子种类。数据表明，1)神经生长因子或碱性成纤维细胞生长因子刺激1分钟后，DGs主要由磷脂酰肌醇水解物产生；2)与之相反，在卡巴胆碱刺激1分钟后，DG由磷脂酰肌醇和磷脂酰胆碱同样产生；3)在任何一种激动剂刺激15分钟后，DGs主要由磷脂酰胆碱水解物产生，少量由磷脂酰胆碱产生。这些结果表明，PC12细胞区分不同激动剂的机制至少有一部分是通过改变磷脂来源和DG生成的动力学来实现的。
We have examined 1,2-diglycerides (DGs) generated in PC12 cells in response to the muscarinic agonist carbachol and compared them with those generated in response to the differentiation factors nerve growth factor and basic fibroblast growth factor. Whereas carbachol stimulates a greater release of inositol phosphates, all three agonists generate similar levels of DGs. In this report, we have analyzed the molecular species of PC12 DGs generated in response to these three agonists. Additionally, we have analyzed the molecular species of PC12 phospholipids. The data indicate that 1) after 1 min of either nerve growth factor or basic fibroblast growth factor stimulation, DGs arise primarily from phosphoinositide hydrolysis; 2) in contrast, after 1 min of carbachol stimulation, DG are generated equally by both phosphoinositide and phosphatidylcholine hydrolysis; and 3) after 15 min of stimulation by any of these agonists, DGs are generated largely by phosphatidylcholine hydrolysis, with a smaller component arising from the phosphoinositides. These results suggest that at least part of the mechanism by which PC12 cells distinguish between different agonists is via alterations in phospholipid sources and kinetics of DG generation.