The transduction of the signal for grape bud dormancy breaking induced by hydrogen cyanamide may involve the SNF-like protein kinase GDBRPK
The transduction of the signal for grape bud dormancy breaking induced by hydrogen cyanamide may involve the SNF-like protein kinase GDBRPK
复制标题
DOI：
10.1023/a:1006450516982
复制
发表时间：
2000-07-01
影响因子：
5.1
通讯作者：
Ogrodovitch, A
中科院分区：
文献类型：
--
作者：
Or, E;Vilozny, I;Ogrodovitch, A
分析了葡萄芽休眠释放早期阶段基因表达的变化，以帮助鉴定可能介导休眠释放信号转导或分生组织活性抑制的基因产物。在本报告中，我们描述了GDBRPK的鉴定，这是一种snf样蛋白激酶的转录本，在氰化氢（HC）的化学诱导下，休眠释放被上调。由于SNF和SNF样蛋白激酶被认为是应激信号的传感器，我们假设GDBRPK可能参与HC诱导的应激信号的感知。我们还描述了在HC应用后不久观察到的PDC和ADH转录本的同时和显著的诱导，并且是短暂的。这些数据可能暗示HC应用导致短暂的呼吸应激，这可能导致AMP/ATP比率暂时增加。由于AMP被认为是一种被snf样激酶感知的应激信号，我们认为snf样GDBRPK可以作为该信号的传感器。
Alterations in gene expression during early stages of dormancy release in grapevine buds were analyzed to facilitate the identification of gene products that may mediate the signal transduction of a dormancy-release signal, or derepression of meristematic activity. In the present report we describe the identification of GDBRPK, a transcript for an SNF-like protein kinase that is up-regulated upon chemical induction of dormancy release by hydrogen cyanamide (HC). Since SNF and SNF-like protein kinases are known as sensors of stress signals, we hypothesize that GDBRPK may be involved in the perception of a stress signal induced by HC. We also describe a simultaneous and remarkable induction of both PDC and ADH transcripts that was observed shortly after HC application, and was of a transient nature. These data may imply that HC application leads to a transient respiratory stress, which likely results in a temporary increase in the AMP/ATP ratio. Since AMP is known as a stress signal that is sensed by SNF-like kinases, we suggest that the SNF-like GDBRPK could serve as the sensor of this signal.