Nitric Oxide-Releasing Emulsion with Hyaluronic Acid and Vitamin E.
Nitric Oxide-Releasing Emulsion with Hyaluronic Acid and Vitamin E.
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含有透明质酸和维生素 E 的一氧化氮释放乳液。
DOI：
10.1039/c9ra03840j
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发表时间：
2019
期刊：
影响因子：
3.9
通讯作者：
Reynolds,MelissaM
中科院分区：
文献类型：
--
作者：
Yapor,JanetP;Gordon,JennaL;Henderson,ChristinaN;Reynolds,MelissaM
S-亚硝基谷胱甘肽（GSNO）是一种天然可用的S-亚硝基硫醇，可掺入用于局部使用的无毒制剂中。一氧化氮（NO）的局部递送价值与其充分研究的生理功能有关，如血管舒张、血管生成、细胞增殖和广谱抗菌活性。先前报道的局部NO释放底物包括对皮肤组织表现出无毒行为的聚合物材料，如聚乙二醇。然而，它们既不能作为保湿剂，也不能为皮肤提供维生素。在本研究中，将GSNO加入到用α-生育酚乙酸酯（维生素E）和透明质酸强化的乳剂中。在150 °C时，NO释放乳剂的平均总NO含量为58 ± 8 μmol g−1，在生理温度（37.4 °C）下53 h内的累积NO释放量为46 ± 4 μmol g−1。对洗剂中的GSNO浓度进行优化，以达到与人皮肤相似的pH值（pH 5.5）。使用旋转粘度计分析S-亚硝化和非亚硝化乳液的粘度，以获得可容易地铺展在皮肤组织上的材料。获得的粘度值范围为7.88 ± 0.99至8.50 ± 0.36 Pa s。先前的研究已经确定洗剂的粘度最大值为100 Pa s。低粘度增加了活性成分向皮肤的扩散系数，因为它们如Einstein-Smoluchowski方程所述成反比。使用比色细胞活力试剂盒I-WST-8，与未处理的HDF相比，评估S-亚硝化和非亚硝化乳剂对成人皮肤成纤维细胞（HDF）的作用。研究结果表明，S-亚硝化或非亚硝化乳剂在HDF中均不诱导细胞毒性。
S-Nitrosoglutathione (GSNO) is a naturally available S-nitrosothiol that can be incorporated into non-toxic formulations intended for topical use. The value of nitric oxide (NO) delivered topically relates to its well-studied physiological functions such as vasodilation, angiogenesis, cell proliferation and broad-spectrum antibacterial activity. Previously reported topical NO-releasing substrates include polymeric materials that exhibit non-toxic behaviors on dermal tissue such as polyethylene glycol. However, they do not serve as humectants nor provide vitamins to the skin. In this study, GSNO was added to an emulsion that was fortified with α-tocopheryl acetate (vitamin E) and hyaluronic acid. The average total NO content for the NO-releasing emulsion was 58 ± 8 μmol g−1 at 150 °C and the cumulative NO release over 53 h at physiological temperature (37.4 °C) was 46 ± 4 μmol g−1. The GSNO concentration in the lotion was optimized in order to reach a pH value similar to that of human skin (pH 5.5). The viscosity was analyzed using a rotational viscometer for the S-nitrosated and the non-nitrosated emulsions to obtain a material that can be readily spread on dermal tissue. The viscosity values obtained ranged from 7.88 ± 0.99 to 8.50 ± 0.36 Pa s. Previous studies have determined that the viscosity maximum for lotions is 100 Pa s. A low viscosity increases the diffusion coefficient of active ingredients to the skin given that they are inversely proportional as described by the Einstein–Smoluchowski equation. The effect of the S-nitrosated and non-nitrosated emulsions on adult human dermal fibroblasts (HDFs) was assessed in comparison to untreated HDFs using Colorimetric Cell Viability Kit I-WST-8. The findings indicate that neither the S-nitrosated nor non-nitrosated emulsions induced cytotoxicity in HDFs.