High contrast two-photon imaging of fingermarks.
High contrast two-photon imaging of fingermarks.
复制标题
指纹的高对比度双光子成像。
DOI：
10.1038/srep24142
复制
发表时间：
2016
影响因子：
4.6
通讯作者：
Rebane,Aleksander
中科院分区：
文献类型：
--
作者：
Stoltzfus,CalebR;Rebane,Aleksander
光学获取的指纹被广泛用作执法机构和法庭的证据。一种常见的技术，用于可视化潜在的指纹无孔表面上的氰基丙烯酸酯发烟的指纹材料，然后浸渍与荧光染料，在紫外线（UV）照射下，使指纹可见，因此可用于数字记录。然而，当图像质量由于高背景散射、衬底材料的高自发荧光或其他有害的光物理和光化学效应（诸如对样品的光诱导损伤）而受到损害时，存在关键情况。在这里，我们提出了一种新的近红外（NIR），双光子诱导荧光成像模式，显着提高指纹图像的质量，特别是当从高反射和/或散射表面获得时，同时减少光损伤敏感的法医样本。
Optically-acquired fingermarks are widely used as evidence across law enforcement agencies as well as in the courts of law. A common technique for visualizing latent fingermarks on nonporous surfaces consists of cyanoacrylate fuming of the fingerprint material, followed by impregnation with a fluorescent dye, which under ultra violet (UV) illumination makes the fingermarks visible and thus accessible for digital recording. However, there exist critical circumstances, when the image quality is compromised due to high background scattering, high auto-fluorescence of the substrate material, or other detrimental photo-physical and photo-chemical effects such as light-induced damage to the sample. Here we present a novel near-infrared (NIR), two-photon induced fluorescence imaging modality, which significantly enhances the quality of the fingermark images, especially when obtained from highly reflective and/or scattering surfaces, while at the same time reducing photo-damage to sensitive forensic samples.