Biology and Genome Sequence of Streptococcus mutans Phage M102AD
Biology and Genome Sequence of Streptococcus mutans Phage M102AD
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DOI：
10.1128/aem.07726-11
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发表时间：
2012-04-01
影响因子：
4.4
通讯作者：
Moineau, Sylvain
中科院分区：
文献类型：
--
作者：
Delisle, Allan L.;Guo, Ming;Moineau, Sylvain
M102AD是1993年描述为噬菌体M102的变形链球菌噬菌体的新名称。这一变化是由于对另一种名为M102的变形链球菌噬菌体的基因组分析所必需的，该分析揭示了与本文报道的基因组序列的差异。进一步的宿主范围分析证实，变形链球菌噬菌体M102AD仅感染少数血清c型菌株。噬菌体M102AD对宿主的吸附非常缓慢，不能吸附变形链球菌的e型和f型菌株。c特异性抗血清阻断M102AD吸附。噬菌体M102AD对热处理细胞和胰蛋白酶处理细胞的吸附也同样好，这表明它是碳水化合物受体。唾液和多糖的产生不抑制斑块的形成。该噬菌体基因组由一个线性双链DNA分子组成，分子量为30664 bp， GC含量为39.6%。对基因组末端的分析表明，存在一个长11个核苷酸的3 '悬垂cos位点。生物信息学分析确定了40个开放的阅读框，都在相同的方向。未发现溶原相关基因，表明噬菌体M102AD具有严格的毒力。未发现明显的毒力因子候选基因。质谱法鉴定了病毒粒子结构中的12个蛋白。对比基因组分析显示变形链球菌噬菌体M102AD和M102以及与嗜热链球菌噬菌体关系密切。这项研究还强调了从公认的微生物收集中获得的生物材料进行研究的重要性。
M102AD is the new designation for a Streptococcus mutans phage described in 1993 as phage M102. This change was necessitated by the genome analysis of another S. mutans phage named M102, which revealed differences from the genome sequence reported here. Additional host range analyses confirmed that S. mutans phage M102AD infects only a few serotype c strains. Phage M102AD adsorbed very slowly to its host, and it cannot adsorb to serotype e and f strains of S. mutans. M102AD adsorption was blocked by c-specific antiserum. Phage M102AD also adsorbed equally well to heat-treated and trypsin-treated cells, suggesting carbohydrate receptors. Saliva and polysaccharide production did not inhibit plaque formation. The genome of this siphophage consisted of a linear, double-stranded, 30,664-bp DNA molecule, with a GC content of 39.6%. Analysis of the genome extremities indicated the presence of a 3 '-overhang cos site that was 11 nucleotides long. Bioinformatic analyses identified 40 open reading frames, all in the same orientation. No lysogeny-related genes were found, indicating that phage M102AD is strictly virulent. No obvious virulence factor gene candidates were found. Twelve proteins were identified in the virion structure by mass spectrometry. Comparative genomic analysis revealed a close relationship between S. mutans phages M102AD and M102 as well as with Streptococcus thermophilus phages. This study also highlights the importance of conducting research with biological materials obtained from recognized microbial collections.