Anti-adrenal cellular hypersensitivity in Addison's disease. IV. In vivo and in vitro investigations on the mitochondrial fraction.
Anti-adrenal cellular hypersensitivity in Addison's disease. IV. In vivo and in vitro investigations on the mitochondrial fraction.
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艾迪生氏病的抗肾上腺细胞超敏反应。
DOI：
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发表时间：
1970
影响因子：
4.6
通讯作者：
G. Bendixen
中科院分区：
文献类型：
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作者：
J. Nerup;V. Andersen;G. Bendixen
对9例特发性Addison病患者和3例糖尿病伴循环抗肾上腺皮质抗体但无Addison病的患者进行了研究。
先前通过白细胞迁移试验在特发性Addison病患者中表现出的器官特异性抗肾上腺细胞超敏反应被发现是针对正常、人、胎儿肾上腺皮质细胞以及人良性增生性肾上腺的线粒体部分。人胎肝细胞的线粒体部分不会引起迁移抑制。
在特发性Addison病患者的淋巴细胞培养中，肾上腺皮质和线粒体部分不能诱导原始细胞转化。因此，这两种体外反应可能表现出不同类型的反应性。
在白细胞对同一抗原的迁移试验呈阳性的患者中，肾上腺皮质线粒体部分能够引发延迟型皮内反应。这一观察证实，抑制白细胞体外迁移表明特发性Addison病患者处于抗肾上腺细胞超敏状态。
Nine patients with idiopathic Addison's disease and three patients with diabetes mellitus and circulating anti-adrenal antibody but without Addison's disease were studied.
The organ-specific, anti-adrenal cellular hypersensitivity previously demonstrated by means of the leucocyte migration test in patients with idiopathic Addison's disease was found to be directed against the mitochondrial fraction of normal, human, foetal adrenocortical cells as well as of human, benign hyperplastic adrenal glands. The mitochondrial fraction of human, foetal liver cells did not cause inhibition of migration.
In lymphocyte cultures from the patients with idiopathic Addison's disease the adrenocortical, mitochondrial fraction did not induce blast transformation. The two in vitro reactions therefore probably express different kinds of reactivity.
The adrenocortical mitochondrial fraction was able to elicit intracutaneous reactions of delayed type in patients with a positive leucocyte migration test to the same antigen. This observation confirms, that inhibition of leucocyte migration in vitro indicates a state of anti-adrenal cellular hypersensitivity in patients with idiopathic Addison's disease.