Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.
Resolution of DNA molecules greater than 5 megabases by contour-clamped homogeneous electric fields.
复制标题
通过轮廓钳制均匀电场分辨率大于 5 兆碱基的 DNA 分子。
DOI：
10.1093/nar/15.19.7865
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发表时间：
1987
影响因子：
14.9
通讯作者：
Davis,RW
中科院分区：
文献类型：
--
作者：
Vollrath,D;Davis,RW
用交变轮廓钳位均匀电场凝胶电泳（CHEF）对酿酒酵母、白色念珠菌和粟酒裂殖酵母的染色体DNA分子进行了高分辨率的分离。这些分子中最大的分子的大小大于5 Mb，并且在1.3 V/cm的场强和1小时的切换间隔下在0.6%琼脂糖凝胶中130小时后被解析。噬菌体λ DNA的多联体的分离揭示了在交替CHEF凝胶电泳中分辨率的四个区域。有两个区域具有良好的分辨率，其中迁移率近似于分子量的线性函数。这些被较低分辨率的区域分开，并且在高分子量处被分辨率很小或没有分辨率的区域限制。这四个区域具有实际的重要性，而且可能具有理论上的重要性。
Excellent resolution of chromosomal DNA molecules fromSaccharomyces cerevisiae, Candida albicansandSchizosaccharomyces pombehas been obtained using alternating contour-clamped homogeneous electric field (CHEF) gel electrophoresis. The largest of these molecules is greater than 5 Mb in size and is resolved after 130 hours in a 0.6% agarose gel at a field strength of 1.3 V/cm and a switching interval of 1 hour. Separation of concatamers of phage lambda DNA reveals four regions of resolution in alternating CHEF gel electrophoresis. There are two regions of good resolution in which mobility approximates a linear function of molecular weight. These are separated by a region of lower resolution and bounded at high molecular weights by a region of little or no resolution. The four regions are of practical and possibly theoretical importance.