Inflammation boosts bacteriophage transfer between Salmonella spp.
Inflammation boosts bacteriophage transfer between Salmonella spp.
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DOI：
10.1126/science.aaf8451
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发表时间：
2017-03-17
期刊：
影响因子：
56.9
通讯作者：
Hardt, Wolf-Dietrich
中科院分区：
文献类型：
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作者：
Diard, Mederic;Bakkeren, Erik;Hardt, Wolf-Dietrich
噬菌体转移（溶原性转化）促进细菌毒力进化。有有限的了解的因素，决定溶原性转换动力学感染宿主。采用鼠伤寒沙门氏菌（S.Tm）腹泻模型研究SopEF（S.Tm SL1344的前噬菌体）向S.Tm ATCC 14028 S的转移。肠道炎症和肠道疾病在3天内触发>55%的ATCC 14028S溶原性转化。在没有炎症的情况下，SopEF转移减少多达10（5）倍。这是因为炎症（例如，活性氧类、活性氮类、次氯酸盐）触发细菌SOS应答，增强噬菌体抗阻遏物Tum的表达，从而促进游离噬菌体的产生和随后的转移。粘液素疫苗接种防止了密集的肠道S.Tm群体诱导炎症，从而消除了SopEF转移。疫苗接种可能是阻断病原体进化的一般策略，其需要温和噬菌体的疾病驱动转移。
Bacteriophage transfer (lysogenic conversion) promotes bacterial virulence evolution. There is limited understanding of the factors that determine lysogenic conversion dynamics within infected hosts. Amurine Salmonella Typhimurium (S.Tm) diarrhea model was used to study the transfer of SopEF, a prophage from S.Tm SL1344, to S.Tm ATCC14028S. Gut inflammation and enteric disease triggered >55% lysogenic conversion of ATCC14028S within 3 days. Without inflammation, SopEF transfer was reduced by up to 10(5)-fold. This was because inflammation (e.g., reactive oxygen species, reactive nitrogen species, hypochlorite) triggers the bacterial SOS response, boosts expression of the phage antirepressor Tum, and thereby promotes free phage production and subsequent transfer. Mucosal vaccination prevented a dense intestinal S.Tm population from inducing inflammation and consequently abolished SopEF transfer. Vaccination may be a general strategy for blocking pathogen evolution that requires disease-driven transfer of temperate bacteriophages.