Nutritional background changes the hypolipidemic effects of fenofibrate in Nile tilapia (Oreochromis niloticus).
Nutritional background changes the hypolipidemic effects of fenofibrate in Nile tilapia (Oreochromis niloticus).
复制标题
营养背景改变非诺贝特对尼罗罗非鱼（Oreochromis niloticus）的降血脂作用
DOI：
10.1038/srep41706
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发表时间：
2017-01-31
影响因子：
4.6
通讯作者：
Du ZY
中科院分区：
文献类型：
--
作者：
Ning LJ;He AY;Lu DL;Li JM;Qiao F;Li DL;Zhang ML;Chen LQ;Du ZY
过氧化物酶体增殖激活受体α（Peroxisome proliferation activated receptor α，PPARα）是一种重要的脂质代谢转录调节因子，在高脂饮食（high fat diet，HFD）和贝特类药物作用下被激活。然而，营养背景是否影响过氧化物酶体增殖物激活体α激活和降血脂作用的过氧化物酶体增殖物激活体α配体还没有研究在鱼类。在目前对尼罗罗非鱼（Oreochromis niloticus）的两阶段研究中，首先用HFD（13%脂肪）或低脂饲料（LFD; 1%脂肪）饲料喂养鱼10周，然后用HFD或LFD喂养第一阶段的鱼补充200毫克/公斤体重的非诺贝特4周。结果表明，HFD不激活PPARα等脂质代谢相关基因。当外源性底物提供充足时，HFD和LFD组在非诺贝特治疗后肝脂肪酸β-氧化显著增加。仅在HFD组中，非诺贝特显著增加肝脏PPARα mRNA和蛋白表达，降低肝脏和血浆甘油三酯浓度。这是第一项研究表明，体脂沉积和饲料脂质含量影响鱼类中的PPARα激活和非诺贝特的降血脂作用，这可能是由于不同饲料喂养的鱼类中脂质catalysts的底物可用性存在差异。
Peroxisome proliferation activated receptor α (PPARα) is an important transcriptional regulator of lipid metabolism and is activated by high-fat diet (HFD) and fibrates in mammals. However, whether nutritional background affects PPARα activation and the hypolipidemic effects of PPARα ligands have not been investigated in fish. In the present two-phase study of Nile tilapia (Oreochromis niloticus), fish were first fed a HFD (13% fat) or low-fat diet (LFD; 1% fat) diet for 10 weeks, and then fish from the first phase were fed the HFD or LFD supplemented with 200 mg/kg body weight fenofibrate for 4 weeks. The results indicated that the HFD did not activate PPARα or other lipid catabolism-related genes. Hepatic fatty acid β-oxidation increased significantly in the HFD and LFD groups after the fenofibrate treatment, when exogenous substrates were sufficiently provided. Only in the HFD group, fenofibrate significantly increased hepatic PPARα mRNA and protein expression, and decreased liver and plasma triglyceride concentrations. This is the first study to show that body fat deposition and dietary lipid content affects PPARα activation and the hypolipidemic effects of fenofibrate in fish, and this could be due to differences in substrate availability for lipid catabolism in fish fed with different diets.