Induction of a bZIP type transcription factor and amino acid catabolism-related genes in soybean seedling in response to starvation stress.
Induction of a bZIP type transcription factor and amino acid catabolism-related genes in soybean seedling in response to starvation stress.
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大豆幼苗响应饥饿胁迫时诱导 bZIP 型转录因子和氨基酸分解代谢相关基因。
DOI：
10.1155/2013/935479
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发表时间：
2013
期刊：
影响因子：
--
通讯作者：
M.
中科院分区：
文献类型：
--
作者：
Yuasa;T.;Nagasawa;K.;Osanai;A.;Kaneko;A.;Tajima;D.;Nang;MPSH.;Ishibashi;Y. and Iwaya-Inoue;M.
为了解决bZIP转录因子在自噬诱导的植物细胞中对氨基酸催化剂的调节作用，我们研究了营养饥饿对大豆（Glycine max（L.）Merr.）。蔗糖饥饿处理显著提高了ZIP53A的表达，但对ZIP53B天冬酰胺合酶（GmASN1）、脯氨酸转氨酶1（GmProDH）和支链氨基酸转氨酶3（GmBCAT 3）的表达没有影响。在蔗糖饥饿和蛋白酶抑制剂的严重饥饿下，ZIP53相关的免疫反应性信号上调，而3%蔗糖和蔗糖饥饿对信号没有影响或影响甚微。不同营养条件下GmASN1、GmProD和GmBCAT 3的诱导曲线与BZIP53蛋白水平曲线一致，但与BZIP53 mRNA水平曲线不一致。这些结果表明，在严重的饥饿胁迫下，GmZIP53蛋白的水平受到翻译后机制的调节，并且在严重的饥饿下，GmZIP53蛋白的增加加速了GmASN 1、GmProDH和GmBCAT 3的转录诱导。此外，可以想象，通过BCAT介导的降解降低支链氨基酸水平最终会增强严重饥饿下的自噬。
To address roles of bZIP transcription factors on regulation of amino acid catabolism under autophagy‐induced plant cells, we examined the effect of nutrient starvation on the expression of low energy stress‐related transcription factor homologs,GmbZIP53A and GmbZIP53B, and amino acid catabolism‐related genes in soybean (Glycine max(L.) Merr.). Sucrose starvation treatment significantly enhanced the expressions ofGmbZIP53A, but notGmbZIP53B asparagine synthase(GmASN1),proline dehydrogenase1(GmProDH), andbranched chain amino acid transaminase 3(GmBCAT3). GmbZIP53‐related immunoreactive signals were upregulated under severe starvation with sucrose starvation and protease inhibitors, while 3% sucrose and sucrose starvation had no or marginal effects on the signal. Profiles of induction ofGmASN1, GmProDHandGmBCAT3under various nutrient conditions were consistent with the profiles of GmbZIP53 protein levels but not with those of GmbZIP mRNA levels. These results indicate that GmbZIP53 proteins levels are regulated by posttranslational mechanism in response to severe starvation stress and that the increased protein of GmbZIP53 under severe starvation accelerates transcriptional induction ofGmASN1,GmProDH, andGmBCAT3. Furthermore, it is conceivable that decrease of branched chain amino acid level by the BCAT‐mediated degradation eventually enhances autophagy under severe starvation.