De novo transcriptome analysis of Thitarodes jiachaensis before and after infection by the caterpillar fungus, Ophiocordyceps sinensis
De novo transcriptome analysis of Thitarodes jiachaensis before and after infection by the caterpillar fungus, Ophiocordyceps sinensis
复制标题
冬虫夏草感染前后甲柴丝霉的从头转录组分析
DOI：
10.1016/j.gene.2016.01.007
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发表时间：
2016-04-12
期刊：
影响因子：
3.5
通讯作者：
Liu, Xin
中科院分区：
文献类型：
--
作者：
Li, Shaosong;Zhong, Xin;Liu, Xin
甲草虫（Thitarodes jiachaensis）是致病性冬虫夏草（Ophiocordyceps sinensis）的宿主，是一种具有广泛药用价值和巨大经济价值的真菌。目前，有关拟虫的基因组信息仍然有限，寄主拟虫幼虫与中华夜蛾在感染过程中的相互作用也不完全清楚。在本研究中，我们利用Illumina测序平台对中华按蚊感染前后的家查按蚊进行转录组测序，并鉴定出与中华按蚊感染后的防御反应相关的转录本。从26.62 gb的clean reads中共获得了161,804个转录本和94,827个unique，其中35.03%的unique在现有数据库中得到了注释。1581个基因在感染后表达显著改变；其中上调928个（58.70%），下调653个（41.30%）。研究人员预测，家蚕对中华白僵虫感染的反应可能与角质层蛋白和营养基质蛋白、抗菌肽、模式识别受体（PRRs）和蛋白水解级联酶等物理屏障基因有关。综上所述，这些数据为进一步研究食虫虫提供了宝贵的基因组资源，并增加了我们对食虫虫与中华弓形虫之间发生的宿主-病原体相互作用的理解。(C) 2016 Elsevier B.V.版权所有
Thitarodes jiachaensis is a host species of the pathogenic caterpillar fungus Ophiocordyceps sinensis, which is a fungus with broad medical effects and great economic value. Curated genomic information on Thitarodes is still limited, and the interaction between host Thitarodes larvae and O. sinensis during infection is incompletely understood. In this study, we performed transcriptome sequencing for T. jiachaensis before and after O. sinensis infection using the Illumina sequencing platform, and we identified the transcripts associated with the defense response of T. jiachaensis upon O. sinensis infection. A total of 161,804 transcripts and 94,827 unigenes for T. jiachaensis were obtained from 26.62-Gb clean reads, and 35.03% of all the unigenes were annotated in current databases. The expression of 1581 genes was significantly altered following infection; among them, 928 (58.70%) were up-regulated and 653 (41.30%) were down-regulated. Genes encoding physical barriers such as cuticle proteins and peritrophic matrix proteins, antimicrobial peptides (AMPs), pattern recognition receptors (PRRs), and enzymes in the proteolytic cascade were predicted to be involved in the response of T. jiachaensis to O. sinensis infection. Together, these data provide a valuable genomic resource for further studies of Thitarodes and increase our understanding of the host-pathogen interaction that occurs between Thitarodes and O. sinensis. (C) 2016 Elsevier B.V. All rights reserved.