A study of the rat neuropeptide B/neuropeptide W system using in situ techniques
A study of the rat neuropeptide B/neuropeptide W system using in situ techniques
复制标题
DOI：
10.1002/cne.20989
复制
发表时间：
2006-07-20
影响因子：
2.5
通讯作者：
Civelli, Olivier
中科院分区：
文献类型：
--
作者：
Jackson, Valerie R.;Lin, Steven H.;Civelli, Olivier
在大鼠中，神经肽B/神经肽W（NPB/NPW）系统由两个配体（神经肽B（NPB）和神经肽W（NPW））和一个受体（GPR 7）组成。尽管初步分析显示了在进食、激素分泌和镇痛中的作用，但缺乏详细的解剖图削弱了我们对NPB/NPW系统的理解。我们证明在这份报告中的表达模式GPR 7，NPB，和NPW前体信使核糖核酸（mRNA）在大鼠脑中使用原位杂交和原位结合实验。杏仁核表达最高水平的GPR 7 mRNA和结合信号。具有高水平表达和结合的其他核是视交叉上核和腹侧结节乳头核。中等水平见于背侧虹膜内膜、背侧覆盖带、床核和红核。在嗅球、丘脑旁核、下丘脑、背外侧被盖、上级丘、蓝斑和孤束核中含量较低。虽然NPB前体主要在脑中以低水平表达，但在前嗅核、梨状皮质、正中视前核、基底外侧杏仁核、海马、内侧结节核、黑质、中缝背核、Edinger-Westphal核和蓝斑中观察到中度表达。结果未检测到NPW前体蛋白的表达。我们的研究大大扩展了先前报道的初步原位数据。有了这张大鼠大脑中NPB/NPW系统的地图，现在可以更好地理解该系统在各种行为范式中的功能含义。
In the rat, the neuropeptide B/neuropeptide W (NPB/NPW) system is composed of two ligands, neuropeptide B (NPB) and neuropeptide W (NPW), and one receptor, GPR7. Although preliminary analyses show roles in feeding, hormone secretion, and analgesia, the lack of a detailed anatomical map impairs our understanding of the NPB/NPW system. We demonstrate in this report the expression patterns of GPR7, NPB, and NPW precursor messenger ribonucleic acid (mRNA) in the rat brain by using in situ hybridization and in situ binding experiments. The amygdala expresses the highest levels of GPR7 mRNA and binding signals. Other nuclei with high levels of expression and binding are the suprachiasmatic and the ventral tuberomamillary nuclei. Moderate levels are seen in the dorsal endopiriform, dorsal tenia tecta, bed nucleus, and the red nucleus. Low levels are in the olfactory bulb, parastrial nucleus, hypothalamus, laterodorsal tegmentum, superior colliculus, locus coeruleus, and the nucleus of the solitary tract. Although the NPB precursor is mostly expressed at low levels in the brain, moderate expression is seen in anterior olfactory nucleus, piriform cortex, median preoptic nucleus, basolateral amygdala, hippocampus, medial tuberal nucleus, substantia nigra, dorsal raphe nucleus, Edinger-Westphal nucleus, and the locus coeruleus. To our surprise, the expression of NPW precursor was not detected. Our study greatly expands the preliminary in situ data previously reported. With this map of the NPB/NPW system in the rat brain, a better understanding of the functional implications of the system in various behavioral paradigms is now possible.