Benzo[a]pyrene induces microbiome dysbiosis and inflammation in the intestinal tracts of western mosquitofish (Gambusia affinis) and zebrafish (Danio rerio)
Benzo[a]pyrene induces microbiome dysbiosis and inflammation in the intestinal tracts of western mosquitofish (Gambusia affinis) and zebrafish (Danio rerio)
复制标题
苯并[a]芘诱导西方蚊鱼（Gambusia affinis）和斑马鱼（Danio rerio）肠道微生物群失调和炎症
DOI：
10.1016/j.fsi.2020.06.041
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发表时间：
2020
影响因子：
4.7
通讯作者：
Jixing Zou
中科院分区：
文献类型：
--
作者：
Shaolin Xie;Aiguo Zhou;Nan Xu;Yongyong Feng;Zhengkun Pan;Muhammad Junaid;Jun Wang;Jixing Zou
苯并[a]芘(BaP)是研究最多的致癌多环芳烃(PAHs)之一，在水生生物中具有广泛的毒性作用。在本研究中，将蚊虫和斑马鱼暴露于BaP(100gμg/L)15天。我们利用16S rRNA基因扩增序列分析了蚊虫和斑马鱼的肠道微生物群落，并对肠道组织中的炎症途径相关基因进行了转录图谱分析。我们的结果表明，暴露于BaP后，蚊虫和斑马鱼的微生物群落组成发生了相似的变化。在门水平上，暴露于BaP后，变形杆菌的丰度降低，而菲米特的丰度增加。在属水平上，与对照组(~0.001%v/v)相比，BaP处理组的常见致病葡萄球菌属显著增加。此外，还观察到BaP显著提高了蚊虫和斑马鱼中IL1β的表达水平。暴露于Bap后，IL6、IL8、IL10和difnphi1在斑马鱼中的转录水平显著增加，而在蚊子鱼中的转录水平则没有显著变化。我们的研究结果表明，BaP可以诱导蚊虫和斑马鱼肠道微生物区系的失调和炎症。
Benzo[a]pyrene (BaP) is one of the most well studied carcinogenic polycyclic aromatic hydrocarbons (PAHs) that has been associated with a wide range of toxic effects in aquatic organisms. In the present study, the mosquitofish and zebrafish were exposed to BaP (100 μg/L) for 15 days. We analyzed the intestinal microbial community of mosquitofish and zebrafish using 16S rRNA gene amplicon sequencing and also performed transcriptional profiling of the inflammation pathway related genes in the intestinal tissues. Our results showed that BaP exposure induced similar changes to the composition of microbial community in mosquitofish and zebrafish. At the phylum level, the abundance of Proteobacteria decreased while the abundance of Firmicutes increased following BaP exposure. At the genus level, a common pathogenic genus staphylococcus significantly increased in the BaP treatment groups, compared to the control (DMSO, ~0.001% v/v). In addition, it was observed that BaP significantly increased the mRNA level ofil1βin both mosquitofish and zebrafish. The transcript levels ofil6,il8,il10andifnphi1were significantly increased in zebrafish, however not in mosquitofish, following Bap exposure. Our findings suggest that BaP could induce microbiota dysbiosis and inflammation in the intestine of mosquitofish and zebrafish.