A GENETIC AND MONOCLONAL ANALYSIS OF HIGH-YIELDING REASSORTANTS OF INFLUENZA-A VIRUS USED FOR HUMAN VACCINES
A GENETIC AND MONOCLONAL ANALYSIS OF HIGH-YIELDING REASSORTANTS OF INFLUENZA-A VIRUS USED FOR HUMAN VACCINES
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DOI：
10.1016/s0092-1157(84)80026-8
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发表时间：
1984-01-01
期刊：
影响因子：
--
通讯作者：
DOWNIE, JC
中科院分区：
文献类型：
--
作者：
DOWNIE, JC
Two methods of analysis using monoclonal antibodies and RNA hydridization to characterize variation in the hemagglutinins of 7 high-yielding influenza virus reassortants used for inactivated vaccine production are described. The results show that variants were selected in producing these genetic reassortants. The hemagglutinins of 2 reassortants showed both antigenic and structural differences from their wild-type (wt) parents as detected by the 2 methods of analysis. These variants were more closely related to other subtype strains which had previously been differentiated from the wt parent by postinfection ferret sera and which also had amino acid sequence differences in antigenically significant sites on the HA1 polypeptide chain of the hemagglutinin molecule. The hemagglutinins of 4 of the 7 reassortants showed antigenic differences but no apparent structural differences from their wt parents. The hemagglutinin of only one reassortant was antigenically and structurally identical to its wt parent. The variants could not be reliably distinguished with hyperimmine rabbit serum or immune ferret serum to the wt parent virus. It is thus important to use more discriminatory tests to identify influenza strains corectly. It is also essential for vaccine strains to be as completely characterized as possible. It is considered desirable that both methods of analysis be used to characterize influenza virus reassortant strains.
