Manufacture of liposomes by isopropanol injection: characterization of the method
Manufacture of liposomes by isopropanol injection: characterization of the method
复制标题
通过异丙醇注射制造脂质体：方法的表征
DOI：
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发表时间：
2012
影响因子：
4.4
通讯作者：
B. Frisch
中科院分区：
文献类型：
--
作者：
P. Gentine;A. Bubel;C. Crucifix;L. Bourel;B. Frisch
单层脂质体通常通过将脂质的乙醇溶液快速注射到水性介质中来制备。本研究的目的是通过使用替代溶剂更有效地控制囊泡直径。结果表明，异丙醇注射液是制备脂质体的乙醇注射液的良好替代品。通过改变水相的搅拌速度和异丙醇-脂质溶液的进样流速等工艺参数，可以控制粒径大小。通过这种方法获得的囊泡的直径受磷脂的性质以及脂质浓度的影响较小，比在乙醇注射过程中。此外，囊泡通常较小（约40-210 nm）。通过荧光、31 P-NMR和低温透射电子显微镜对颗粒的准确表征表明，颗粒由围绕水腔的单个脂质双层形成。因此，我们为科学界提供了一个充分表征的替代方法来生产单层囊泡。
Unilamellar liposomes are conventionally prepared by rapid injection of an ethanolic solution of lipids into an aqueous medium. The aim of the present study was to control, more efficiently, vesicle diameter by using an alternative solvent. The results show that isopropanol injection is a good alternative to ethanol injection for the manufacture of liposomes. Particle size can be controlled by the variation of process parameters, such as stirring speed of the aqueous phase and injection flow rate of lipid-isopropanol solution. Diameter of vesicles obtained by this method is less affected by the nature of phospholipid, as well as lipid concentration, than in the ethanol-injection process. In addition, the vesicles are generally smaller (approximately 40–210 nm). Accurate characterization of the particles, by fluorescence, 31P-NMR, and cryo–transmission electron microscopy, showed that particles are formed of a single lipid bilayer around an aqueous cavity. We thus provide the scientific community with a fully characterized alternative method to produce unilamellar vesicles.
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影响因子：
2.9
作者：
Ladokhin, AS;Jayasinghe, S;White, SH
通讯作者：
White, SH
影响因子：
3.4
作者：
Chanturiya, A;Leikina, E;Chernomordik, LV
通讯作者：
Chernomordik, LV
甾醇对缺乏酰基的磷脂酰胆碱双层的渗透性和相变的影响。
影响因子：
2.9
作者：
Bittman,R;Clejan,S;Jain,MK;Deroo,PW;Rosenthal,AF
通讯作者：
Rosenthal,AF
人红细胞膜中磷脂酰胆碱单个分子种类的翻转率。
DOI：
10.1016/0005-2736(86)90087-8
发表时间：
1986
期刊：
Biochimica et biophysica acta
影响因子：
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作者：
Middelkoop,E;Lubin,BH;OpdenKamp,JA;Roelofsen,B
通讯作者：
Roelofsen,B