A DEMONSTRATION OF THE ADVANTAGES OF IMMUNOSTAINING IN THE QUANTIFICATION OF AMYLOID PLAQUE DEPOSITS
A DEMONSTRATION OF THE ADVANTAGES OF IMMUNOSTAINING IN THE QUANTIFICATION OF AMYLOID PLAQUE DEPOSITS
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DOI：
10.1007/bf00500553
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
ROBERTS, GW
中科院分区：
文献类型：
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作者：
GENTLEMAN, SM;BRUTON, C;ROBERTS, GW
细胞外淀粉样蛋白沉积是阿尔茨海默型痴呆和“正常”衰老过程的特征。淀粉样蛋白斑块沉积的定量可能有助于区分与“正常”衰老相关的衰老变化和痴呆的病理过程。为了确定用于淀粉样蛋白可视化的最可靠和可再现的方法，我们将常规银染色技术与β-淀粉样蛋白染色技术进行了比较。在来自痴呆患者（n = 15，年龄范围60-87）和非痴呆患者（n = 65，年龄范围14-99）的大样本的港口尸检脑组织上进行淀粉样蛋白免疫细胞化学。淀粉样蛋白沉积的程度用四点量表评定，两种技术的评分显著相关（P < 0.01）。然而，免疫细胞化学方法在定量方面具有许多明显的优势。针对β的抗体淀粉样蛋白是高度特异性的，并且不染色神经纤维缠结或背景特征，在突出弥散的淀粉样蛋白沉积物方面，它比银染色敏感得多，并且可能最重要的是，它产生高的淀粉样蛋白染色，这允许更容易的图像数字化和随后的计算机图像分析。
Extracellular amyloid deposits are a feature of both Alzheimer type dementia and the ''normal'' aging process. Quantification of amyloid plaque deposits may well be useful in distinguishing between the senescent changes associated with ''normal'' aging and the pathological processes underlying dementia. To determine the most reliable and reproducible method for visualisation of the amyloid we have compared conventional silver staining techniques with .beta.-amyloid immunocytochemistry on a large sample of port-mortem brain tissue from both demented (n = 15, age range 60-87) and non-demented (n = 65, age range 14-99) patients. The degree of amyloid deposition was rated on a four point scale and ratings for the two techniques were significantly correlated (P < 0.01). However, the immunocytochemical approach has a number of distinct advantages for quantification. The antibody to .beta.-amyloid is highly specific and does not stain neurofibrillary tangles or background features, it is considerably more sensitive than silver staining in highlighting diffuse amyloid deposits and, perhaps most importantly, it produces high constrast staining which allows easier image digitisation and subsequent computer image analysis.