2 NOVEL MONOCLONAL-ANTIBODIES TO FIBRONECTIN THAT RECOGNIZE THE HEP-II AND CS-1 REGIONS RESPECTIVELY - THEIR DIFFERENTIAL EFFECT ON LYMPHOCYTE ADHESION
2 NOVEL MONOCLONAL-ANTIBODIES TO FIBRONECTIN THAT RECOGNIZE THE HEP-II AND CS-1 REGIONS RESPECTIVELY - THEIR DIFFERENTIAL EFFECT ON LYMPHOCYTE ADHESION
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DOI：
10.1016/s0006-291x(05)80785-5
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发表时间：
1992-07-15
影响因子：
3.1
通讯作者：
WAYNER, EA
中科院分区：
文献类型：
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作者：
GARCIAPARDO, A;SANCHEZAPARICIO, P;WAYNER, EA
我们已经获得了两个新的单克隆抗体的纤维连接蛋白的羧基末端区域，即P3 D4和P1 F11，并研究了它们的结合位点和它们的能力，以阻止淋巴细胞粘附到纤维连接蛋白。ELISA和Western blot分析表明，P3 D4与两条纤连蛋白链和两个含Hep II的片段（58 kDa和38 kDa）反应。针对合成肽CS-1产生的P1 F11与来自纤连蛋白A链的38 kDa片段和190 kDa片段反应。P1 F11不与58 kDa片段反应，因此清楚地确定58 kDa来自纤连蛋白的B链并且缺乏CS-1序列。使用mAb P3 D4和P1 F11来评估Hep II和CS-1位点在细胞附着至纤连蛋白中的贡献。P3 D4有效地抑制B细胞粘附到38 kDa，58 kDa和纤连蛋白;然而，P1 F11仅产生有限的抑制，这表明淋巴细胞与Hep II的相互作用可能进一步调节与CS-1位点的结合。
We have obtained two new mAbs to the carboxy-terminal region of fibronectin, namely P3D4 and P1F11, and have studied their binding sites and their ability to block lymphocyte adhesion to fibronectin. ELISA and Western blot analyses showed that P3D4 reacts with both fibronectin chains and both Hep II-containing fragments (58 kDa and 38 kDa). P1F11, raised against the synthetic peptide CS-1, reacted with the 38 kDa fragment and with a 190 kDa fragment derived from the A chain of fibronectin. P1F11 did not react with the 58 kDa fragment thus clearly establishing that 58 kDa comes from the B chain of fibronectin and lacks the CS-1 sequence. mAbs P3D4 and P1 F11 were used to evaluate the contribution of the Hep II and CS-1 sites in cell attachment to fibronectin. P3D4 effectively inhibited B cell adhesion to 38 kDa, 58 kDa and fibronectin; P1 F11 however produced only limited inhibition, suggesting that lymphocyte interaction with Hep II may modulate further binding to the CS-1 site.