THE CENTRAL NUCLEUS OF THE INFERIOR COLLICULUS IN RAT - A GOLGI AND COMPUTER RECONSTRUCTION STUDY OF NEURONAL AND LAMINAR STRUCTURE
THE CENTRAL NUCLEUS OF THE INFERIOR COLLICULUS IN RAT - A GOLGI AND COMPUTER RECONSTRUCTION STUDY OF NEURONAL AND LAMINAR STRUCTURE
复制标题
DOI：
10.1002/cne.903330102
复制
发表时间：
1993-07-01
影响因子：
2.5
通讯作者：
MOLOWNY, RL
中科院分区：
文献类型：
--
作者：
MALMIERCA, MS;BLACKSTAD, TW;MOLOWNY, RL
本研究采用计算机辅助三维重建的方法，对成年大鼠树脂包埋材料连续切片中高尔基体浸渍的树突状结构进行了研究，探讨了下丘中央核层状结构的细胞学基础。两种类型的扁平神经元，这里定义为扁平（F）和不太扁平（LF），被描述为有助于模式。F神经元树突的绝对厚度较小（平均约50 μ m），树突的密度较大。它们在区域上惊人地平行，形成的纹层大多只有一个细胞厚。板层似乎被LF神经元填充的层间室分开。后者的乔木较厚（平均约100妈妈），密度低于F神经元。这两种类型的不同密度可能，至少部分地，是负责在层和层间室内的定向树突的密度的相应差异。F和LF心轴的定向平面大致相似，但不排除F和LF心轴之间的定向存在一致的轻微差异。大多数的F和LF乔木延长平行于腹外侧背内侧取向的长轴的椎板。少数是面向喙尾或中间方向。层间室在低频区比在高频区显得不那么明显。后者的区域也不同于前者的F神经元具有较高数量的中间和终末段和更致密的乔木。它是讨论所观察到的F和LF细胞是否构成两种不同的细胞类型或一种类型的神经元的品种，与形态上的差异反映了不同的位置。要解决这一问题，还需要在组织化学、hodological和其他标准上对神经元进行进一步表征。
The cellular basis for the laminar structure of the central nucleus of the inferior colliculus has been investigated by computer-assisted 3-D reconstruction of Golgi impregnated dendritic arbors, sampled from serial sections of resin embedded material of adult rat. Two types of flattened neurone, defined here as flat (F) and less flat (LF), are described as contributing to the pattern. The dendritic arbors of F neurones had a smaller absolute thickness (mean about 50 mum) and a denser arbor. They were strikingly parallel regionally and formed laminae mostly one cell thick. The laminae appeared to be separated by interlaminar compartments populated by the LF neurones. The arbors of the latter were thicker (mean about 100 mum) and less dense than those of the F neurones. The different density of the two types may, at least in part, be responsible for the corresponding difference in density of oriented dendrites within the laminae and interlaminar compartments. The orientation planes of F and LF arbors were roughly similar, but a consistent, slight difference in orientation between F and LF arbors is not excluded. Most of the F and LF arbors were elongated in parallel with the ventrolaterally to dorsomedially oriented long axis of the laminae. A few were instead oriented rostrocaudally or in intermediate directions. The interlaminar compartments appeared less distinct in the low than in the high frequency region. The latter region also differed from the former by having F neurones with a higher number of intermediate and terminal segments and a denser arbor. It is discussed whether the observed F and LF cells constitute two distinct cell types or are varieties of one type of neurone, with the morphological differences reflecting differences in location. Further characterization of the neurones on histochemical, hodological, and other criteria is required to settle this question.