A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast
A cell cycle-regulated GATA factor promotes centromeric localization of CENP-A in fission yeast
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DOI：
10.1016/s1097-2765(03)00011-x
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发表时间：
2003-01-01
期刊：
影响因子：
16
通讯作者：
Takahashi, K
中科院分区：
文献类型：
--
作者：
Chen, ES;Saitoh, S;Takahashi, K
CENP-A是着丝粒特异性组蛋白H3变体，在组织着丝粒染色质以实现染色体的精确分离中起着至关重要的作用。我们已经分离出含有GATA因子的daxx样基序Ams2和组蛋白H4，作为pombe S. CENP-A突变体cnp1-1的多拷贝抑制因子。虽然Ams2的缺失导致CENP-A与着丝粒结合减少和染色体错分离，但增加其剂量可以恢复CENP-A突变蛋白与着丝粒的关联。相反，过表达CENP-A或组蛋白H4会抑制ams2干扰物。因此，胞内Ams2的量影响着着丝粒核小体成分。Ams2在S期丰富，并通过结合gata核心序列与染色质结合，包括中央着丝粒。因此，Ams2是一个细胞周期调节的GATA因子，是着丝粒功能所必需的。
CENP-A, the centromere-specific histone H3 variant, plays a crucial role in organizing kinetochore chromatin for precise chromosome segregation. We have isolated Ams2, a Daxx-like motif-containing GATA factor, and histone H4, as multicopy suppressors of cnp1-1, an S. pombe CENP-A mutant. While depletion of Ams2 results in the reduction of CENP-A binding to the centromere and chromosome missegregation, increasing its dosage restores association of a CENP-A mutant protein with centromeres. Conversely, overexpression of CENP-A or histone H4 suppresses an ams2 disruptant. The intracellular amount of Ams2 thus affects centromeric nucleosomal constituents. Ams2 is abundant in S phase and associates with chromatin, including the central centromeres through binding to GATA-core sequences. Ams2 is thus a cell cycle-regulated GATA factor that is required for centromere function.