Platelet derived growth factor (PDGF) contained in Platelet Rich Plasma (PRP) stimulates migration of osteoblasts by reorganizing actin cytoskeleton
Platelet derived growth factor (PDGF) contained in Platelet Rich Plasma (PRP) stimulates migration of osteoblasts by reorganizing actin cytoskeleton
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DOI：
10.4161/19336918.2014.972785
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发表时间：
2014-11-01
影响因子：
3.2
通讯作者：
Colciago, Alessandra
中科院分区：
文献类型：
--
作者：
Casati, Lavinia;Celotti, Fabio;Colciago, Alessandra
富血小板血浆（PRP）是在少量血浆中浓缩的血小板。它富含能够刺激骨形成细胞迁移和生长的生长因子。PRP通常用于临床应用，如牙科手术和骨折愈合。血小板衍生生长因子（PDGF）在PRP中高度浓缩，并且在我们先前的研究中显示其在微趋化性测定中向SaOS-2成骨细胞提供趋化性刺激以移动。本研究的目的是分析用PRP预处理SaOS-2细胞（短时间过程：30- 150 min）对肌动蛋白细胞骨架组织的影响，肌动蛋白细胞骨架是细胞运动的主要效应物。结果表明，PRP预处理增加趋化作用和趋化性，并伴随诱导肌动蛋白微丝的组织，通过免疫细胞化学可视化，在一个定向伸长的表型，这是细胞能够移动的特征。PRP还产生PGDF受体表达的瞬时增加。这种重组被PDGF的免疫中和作用阻断，证明了这种生长因子在触发负责细胞运动的机制中的作用。
Platelet-rich plasma (PRP) is a platelet concentrate in a small volume of plasma. It is highly enriched in growth factors able to stimulate the migration and growth of bone-forming cells. PRP is often used in clinical applications, as dental surgery and fracture healing. Platelet derived growth factor (PDGF), is highly concentrated in PRP and it was shown in our previous studies to provide the chemotactic stimulus to SaOS-2 osteoblasts to move in a microchemotaxis assay. Aim of the present studies is to analyze the effects of a PRP pretreatment (short time course: 30-150min) of SaOS-2 cells with PRP on the organization of actin cytoskeleton, the main effector of cell mobility. The results indicate that a pretreatment with PRP increases chemokinesis and chemotaxis and concomitantly induces the organization of actin microfilaments, visualized by immunocytochemistry, in a directionally elongated phenotype, which is characteristic of the cells able to move. PRP also produces a transient increase in the expression of PGDF receptor. This reorganization is blocked by the immunoneutralization of PDGF demonstrating the responsibility of this growth factor in triggering the mechanisms responsible for cellular movements.