Assessment of variability in the plasma 7k SomaScan proteomics assay.
Assessment of variability in the plasma 7k SomaScan proteomics assay.
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DOI：
10.1038/s41598-022-22116-0
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发表时间：
2022-10-13
影响因子：
4.6
通讯作者：
中科院分区：
文献类型：
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作者：
SomaScan是一种高通量、基于适配体的蛋白质组学检测方法，用于同时测量数千种具有广泛内源性浓度的蛋白质。在其最新版本中，7k SomaScan检测v4.1能够测量7288种人类蛋白质。在这项工作中，我们基于对22个板块的2050个样本的研究，对该平台进行了广泛的技术评估。研究设计包括来自102名人类受试者的板间技术副本，这使我们能够表征不同的标准化程序，通过多种分析方法评估测定变异性，提出信号-背景指标，并讨论潜在的特异性问题。通过对广泛的技术方面提供详细的性能评估，我们的目标是使这项工作成为SomaScan用户不断增长的社区的宝贵资源。
SomaScan is a high-throughput, aptamer-based proteomics assay designed for the simultaneous measurement of thousands of proteins with a broad range of endogenous concentrations. In its most current version, the 7k SomaScan assay v4.1 is capable of measuring 7288 human proteins. In this work, we present an extensive technical assessment of this platform based on a study of 2050 samples across 22 plates. Included in the study design were inter-plate technical duplicates from 102 human subjects, which allowed us to characterize different normalization procedures, evaluate assay variability by multiple analytical approaches, present signal-over-background metrics, and discuss potential specificity issues. By providing detailed performance assessments on this wide range of technical aspects, we aim for this work to serve as a valuable resource for the growing community of SomaScan users.
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DOI：
10.1038/mtna.2014.49
发表时间：
2014-10-07
期刊：
Molecular therapy. Nucleic acids
影响因子：
--
作者：
通讯作者：
--
影响因子：
4.6
作者：
Candia J;Cheung F;Kotliarov Y;Fantoni G;Sellers B;Griesman T;Huang J;Stuccio S;Zingone A;Ryan BM;Tsang JS;Biancotto A
通讯作者：
Biancotto A
影响因子：
3.7
作者：
Gold L;Ayers D;Bertino J;Bock C;Bock A;Brody EN;Carter J;Dalby AB;Eaton BE;Fitzwater T;Flather D;Forbes A;Foreman T;Fowler C;Gawande B;Goss M;Gunn M;Gupta S;Halladay D;Heil J;Heilig J;Hicke B;Husar G;Janjic N;Jarvis T;Jennings S;Katilius E;Keeney TR;Kim N;Koch TH;Kraemer S;Kroiss L;Le N;Levine D;Lindsey W;Lollo B;Mayfield W;Mehan M;Mehler R;Nelson SK;Nelson M;Nieuwlandt D;Nikrad M;Ochsner U;Ostroff RM;Otis M;Parker T;Pietrasiewicz S;Resnicow DI;Rohloff J;Sanders G;Sattin S;Schneider D;Singer B;Stanton M;Sterkel A;Stewart A;Stratford S;Vaught JD;Vrkljan M;Walker JJ;Watrobka M;Waugh S;Weiss A;Wilcox SK;Wolfson A;Wolk SK;Zhang C;Zichi D
通讯作者：
Zichi D
影响因子：
2
作者：
Tuli L;Tsai TH;Varghese RS;Xiao JF;Cheema A;Ressom HW
通讯作者：
Ressom HW
影响因子：
3.4
作者：
Raffield, Laura M.;Dang, Hong;Bowler, Russell P.
通讯作者：
Bowler, Russell P.