Phage Transduction
Phage Transduction
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DOI：
10.1007/978-1-4939-6361-4_13
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发表时间：
2016-01-01
期刊：
影响因子：
--
通讯作者：
Goh, Shan
中科院分区：
文献类型：
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作者：
Goh, Shan
噬菌体通过一种称为转导的机制介导水平基因转移。在实验室进行的噬菌体转导涉及细菌供体和受体，两者都容易受到目标噬菌体的感染。噬菌体在供体中繁殖、浓缩，并以不同的感染率短暂暴露于受体。通过在选择性培养基上培养来选择具有所需表型的转导子。在这里，我们描述了艰难梭菌噬菌体phi C2对ermB的转导，赋予了对红霉素的抗性。
Bacteriophages mediate horizontal gene transfer through a mechanism known as transduction. Phage transduction carried out in the laboratory involves a bacterial donor and a recipient, both of which are susceptible to infection by the phage of interest. Phage is propagated in the donor, concentrated, and exposed transiently to recipient at different multiplicity of infection ratios. Transductants are selected for the desired phenotype by culture on selective medium. Here we describe transduction of ermB conferring resistance to erythromycin by the C. difficile phage phi C2.