Topography and near-field image measurement of soft biological samples in liquid by using a tuning fork based bent optical-fiber sensor
Topography and near-field image measurement of soft biological samples in liquid by using a tuning fork based bent optical-fiber sensor
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DOI：
10.1063/1.3582662
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发表时间：
2011-04-01
影响因子：
1.6
通讯作者：
Kang, Yongseok
中科院分区：
文献类型：
--
作者：
Kwon, Sangjin;Jeong, Sungho;Kang, Yongseok
报道了一种基于音叉的弯曲光纤传感器的制作及其在生理溶液中软生物样品形貌和近场图像测量中的应用。通过采用弯曲光纤和音叉反馈方案，最大限度地减少了信号受杂散光干扰的可能性，这对于近场应用尤其重要。从所测量的音叉振幅和其校准与MEMS输出电压，它被确定，光纤尖端和液体中的软样品之间的相互作用力需要被控制在约10 nN的水平内，图像质量敏感地依赖于相互作用力。固定的COS-7和MCF-7细胞在磷酸盐缓冲盐水和红细胞的近场成像的分辨率约为100 nm的地形测量的结果也在磷酸盐缓冲盐水。(C)2011年美国物理学会。[doi：10.1063/1.3582662]
The fabrication of a tuning fork based bent optical-fiber sensor and its application for topography and near-field image measurement of soft biological samples in physiological solution are reported. By adopting the bent optical fiber and tuning fork feedback scheme, the possibility of signal interference with stray light is minimized, which is especially important for near-field applications. From the measured tuning fork amplitude and its calibration with the preamplifier output voltage, it was determined that the interaction force between the fiber tip and a soft sample in liquid needs to be controlled within approximately 10 nN level and that the image quality depends sensitively to the interaction force. The results of topography measurements of fixed COS-7 and MCF-7 cells in phosphate buffered saline and of the near-field imaging of red blood cell also in phosphate buffered saline with a resolution of about 100 nm are presented. (C) 2011 American Institute of Physics. [doi:10.1063/1.3582662]