Measuring hemodynamics in the developing heart tube with four-dimensional gated Doppler optical coherence tomography
Measuring hemodynamics in the developing heart tube with four-dimensional gated Doppler optical coherence tomography
复制标题
DOI：
10.1117/1.3509382
复制
发表时间：
2010-11-01
影响因子：
3.5
通讯作者：
Rollins, Andrew M.
中科院分区：
文献类型：
--
作者：
Jenkins, Michael W.;Peterson, Lindsy;Rollins, Andrew M.
血液动力学被认为在心脏发育中起着重要作用，但定量评估胚胎血液动力学的工具非常缺乏。特别具有挑战性的早期胚胎血液动力学分析需要新技术。血流的微小变化可以指示异常何时开始，甚至在结构变化可以被检测到之前。此外，早期胚胎中影响血液流动的微小变化可能导致后期的严重异常。我们提出了一个演示的4-D多普勒光学相干断层扫描（OCT）成像的结构和流量，并提出了几个新的血液动力学测量胚胎禽心脏在早期阶段之前形成的四个腔室。使用4-D数据，脉冲多普勒测量可以准确地实现在流入和流出的心管。此外，通过采用来自4-D多普勒图像集的正面切片，获得每搏输出量和心输出量的测量，而不需要确定绝对速度。最后，使用与血流正交的图像平面，通过计算与内皮细胞垂直的速度梯度来确定剪切应力。血流动力学测量将是至关重要的，以确定遗传和环境因素，导致先天性心脏缺陷。(C)2010年，美国光学仪器工程师学会（Society of Photo-Optical Instrumentation Engineers）[DOI 10.1117/1.3509382]
Hemodynamics is thought to play a major role in heart development, yet tools to quantitatively assess hemodynamics in the embryo are sorely lacking. The especially challenging analysis of hemodynamics in the early embryo requires new technology. Small changes in blood flow could indicate when anomalies are initiated even before structural changes can be detected. Furthermore, small changes in the early embryo that affect blood flow could lead to profound abnormalities at later stages. We present a demonstration of 4-D Doppler optical coherence tomography (OCT) imaging of structure and flow, and present several new hemodynamic measurements on embryonic avian hearts at early stages prior to the formation of the four chambers. Using 4-D data, pulsed Doppler measurements could accurately be attained in the inflow and outflow of the heart tube. Also, by employing an enface slice from the 4-D Doppler image set, measurements of stroke volume and cardiac output are obtained without the need to determine absolute velocity. Finally, an image plane orthogonal to the blood flow is used to determine shear stress by calculating the velocity gradient normal to the endocardium. Hemodynamic measurements will be crucial to identifying genetic and environmental factors that lead to congenital heart defects. (C) 2010 Society of Photo-Optical Instrumentation Engineers. [DOI: 10.1117/1.3509382]