Quantification of Nonproteolytic Clostridium botulinum Spore Loads in Food Materials.
Quantification of Nonproteolytic Clostridium botulinum Spore Loads in Food Materials.
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DOI：
10.1128/aem.03630-15
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发表时间：
2016-01-04
影响因子：
4.4
通讯作者：
Peck MW
中科院分区：
文献类型：
--
作者：
Barker GC;Malakar PK;Plowman J;Peck MW
我们制作了数据并进行了分析，以建立非蛋白水解性肉毒梭菌孢子在英国最低限度加工冷藏食品生产过程中使用的材料中的浓度表示。食品材料被分类为同质组，包括肉、鱼、贝类、谷物、新鲜植物材料、乳制品液体、乳制品非液体、蘑菇和真菌、干草药和香料。模型是在贝叶斯框架中构建的，代表了来自建立检测限的阳性对照实验和真实食品材料的专用微生物测试的孢子负荷文献调查的信息组合。非蛋白水解型肉毒杆菌的检测采用了选择富集培养与多重PCR相结合的优化方案，大多数食品材料检测均为阴性。关于孢子负荷的后验信念集中在1至10孢子kg−1的浓度范围内。大孢子负荷的后验信念对干燥的草药和香料最为显著，并且对对照实验的详细结果最为敏感。孢子负荷的概率分布以一种方便的形式表示，可用于数值分析和风险评估。
We have produced data and developed analysis to build representations for the concentration of spores of nonproteolytic Clostridium botulinum in materials that are used during the manufacture of minimally processed chilled foods in the United Kingdom. Food materials are categorized into homogenous groups which include meat, fish, shellfish, cereals, fresh plant material, dairy liquid, dairy nonliquid, mushroom and fungi, and dried herbs and spices. Models are constructed in a Bayesian framework and represent a combination of information from a literature survey of spore loads from positive-control experiments that establish a detection limit and from dedicated microbiological tests for real food materials. The detection of nonproteolytic C. botulinum employed an optimized protocol that combines selective enrichment culture with multiplex PCR, and the majority of tests on food materials were negative. Posterior beliefs about spore loads center on a concentration range of 1 to 10 spores kg−1. Posterior beliefs for larger spore loads were most significant for dried herbs and spices and were most sensitive to the detailed results from control experiments. Probability distributions for spore loads are represented in a convenient form that can be used for numerical analysis and risk assessments.