Aberrant CpG island methylation of PTEN is an early event in nasopharyngeal carcinoma and a potential diagnostic biomarker
Aberrant CpG island methylation of PTEN is an early event in nasopharyngeal carcinoma and a potential diagnostic biomarker
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PTEN 异常 CpG 岛甲基化是鼻咽癌的早期事件，也是潜在的诊断生物标志物
DOI：
10.3892/or.2014.3061
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发表时间：
2014-05-01
期刊：
影响因子：
4.2
通讯作者：
Peng, Hong
中科院分区：
文献类型：
--
作者：
Li, Jinbang;Gong, Pinggui;Peng, Hong
除了鼻咽癌（NPC）外，在某些类型的肿瘤中也观察到磷酸酶和紧张素同源物（PTEN）因其CpG岛高甲基化而失活。在本研究中，我们重点研究了鼻咽癌中PTEN CpG岛的异常甲基化。采用定量PCR方法检测45例鼻咽癌和22例非肿瘤鼻咽上皮组织中PTEN mRNA的表达。通过甲基化特异性聚合酶链反应和测序检测PTEN的甲基化状态。用5-aza-2′-脱氧胞苷（5-aza-dC）处理3株鼻咽癌细胞株，检测PTEN mRNA的表达。PTEN在NPC组织和NPC细胞系中均下调，NPC标本中PTEN的甲基化水平（82.2%）相对于NP组织（5.3%）较高。5-aza-dC可恢复鼻咽癌细胞株PTEN mRNA的表达。这些结果首次揭示了鼻咽癌的表观遗传改变，即PTEN的异常甲基化，这可能是鼻咽癌的早期事件，可能被视为早期检测和预防鼻咽癌的一种新的候选生物标志物。
The inactivation of phosphatase and tensin homolog (PTEN) due to its CpG island hypermethylation has been observed in some types of tumors except nasopharyngeal carcinoma (NPC). In the present study, we focused on the aberrant methylation of PTEN CpG islands in NPC. The mRNA expression of PTEN was detected by quantitative PCR in 45 NPC and 22 non-tumor nasopharyngeal epithelial (NP) tissues. The methylation status of PTEN was examined by methylation-specific polymerase chain reaction and sequencing. The mRNA expression of PTEN in three NPC cell lines treated with 5-aza-2'-deoxycytidine (5-aza-dC) was also examined. PTEN was downregulated in both NPC tissues and NPC cell lines and a relatively higher methylation level of PTEN was found in NPC specimens (82.2%) relative to NP tissues (5.3%). The PTEN mRNA expression was restored in NPC cell lines by treatment with 5-aza-dC. These results first reveal an epigenetic alteration, aberrant methylation of PTEN, in NPC, which is probably an early event and may be regarded as a novel candidate biomarker for early stage of NPC detection and prevention.