Rice NIN-LIKE PROTEIN 1 rapidly responds to nitrogen deficiency and improves yield and nitrogen use efficiency
Rice NIN-LIKE PROTEIN 1 rapidly responds to nitrogen deficiency and improves yield and nitrogen use efficiency
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DOI：
10.1093/jxb/eraa292
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发表时间：
2020-10-07
影响因子：
6.9
通讯作者：
Xiang, Cheng-Bin
中科院分区：
文献类型：
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作者：
Alfatih, Alamin;Wu, Jie;Xiang, Cheng-Bin
氮素是作物生长和产量所必需的，但过量施用氮肥已产生严重的环境问题。科普这些问题的理想且经济的解决方案是提高作物氮素利用效率（NUE）。植物NUE一直是世界范围内深入研究的焦点，但仍有很多关于其遗传决定因素和调控的研究。在这里，我们表明，水稻（Oryza sativa L.）类氮蛋白1（OsNLP 1）在氮素利用中起着重要作用。OsNLP 1蛋白定位于细胞核内，其转录水平被N饥饿迅速诱导。OsNLP 1的过表达在不同氮素条件下提高了植物生长、籽粒产量和氮素利用效率，而OsNLP 1的敲除在氮素限制条件下降低了籽粒产量和氮素利用效率。OsNLP 1通过协调多个氮吸收和同化基因来调控硝酸盐和铵的利用。染色质免疫沉淀和酵母单杂交实验表明，OsNLP 1可以直接与这些基因的启动子结合，激活其表达。因此，我们的结果表明OsNLP 1是氮利用的关键调节因子，并且代表了提高水稻NUE和产量的潜在目标。
Nitrogen (N) is indispensable for crop growth and yield, but excessive agricultural application of nitrogenous fertilizers has generated severe environmental problems. A desirable and economical solution to cope with these issues is to improve crop nitrogen use efficiency (NUE). Plant NUE has been a focal point of intensive research worldwide, yet much still has to be learned about its genetic determinants and regulation. Here, we show that rice (Oryza sativa L.) NIN-LIKE PROTEIN 1 (OsNLP1) plays a fundamental role in N utilization. OsNLP1 protein localizes in the nucleus and its transcript level is rapidly induced by N starvation. Overexpression of OsNLP1 improves plant growth, grain yield, and NUE under different N conditions, while knockout of OsNLP1 impairs grain yield and NUE under N-limiting conditions. OsNLP1 regulates nitrate and ammonium utilization by cooperatively orchestrating multiple N uptake and assimilation genes. Chromatin immunoprecipitation and yeast one-hybrid assays showed that OsNLP1 can directly bind to the promoter of these genes to activate their expression. Therefore, our results demonstrate that OsNLP1 is a key regulator of N utilization and represents a potential target for improving NUE and yield in rice.