Development of a mixed-species biofilm model and its virulence implications in device related infections
Development of a mixed-species biofilm model and its virulence implications in device related infections
复制标题
DOI：
10.1002/jbm.b.34103
复制
发表时间：
2019-01-01
影响因子：
3.4
通讯作者：
Jenkins, A. Toby A.
中科院分区：
文献类型：
--
作者：
Thet, Naing T.;Wallace, Laura;Jenkins, A. Toby A.
越来越多的人认为，为了理解和模拟非生物表面的细菌定植和感染，需要使用生物膜模型。存在至少两种主要物种的许多细菌定殖，然而这难以在体外建模。本研究报告了一个简单的混合物种的生物膜模型的发展，使用菌株的两个临床上显着的细菌：金黄色葡萄球菌和铜绿假单胞菌生长在纳米多孔聚碳酸酯膜上的营养琼脂支持。扫描电子显微镜显示了两种细菌混合在广泛的细胞外基质中的复杂生物膜特征。使用原型伤口敷料，检测溶细胞毒力因子，30个单一和40个混合物种的生物膜的毒力分泌进行了测试。铜绿假单胞菌的竞争力优于S。金黄色葡萄球菌，导致以铜绿假单胞菌为主的生物膜。原型敷料下的混合物种生物膜的原位生长显示了活生物膜群体与其相关毒力因子之间的实时相关性，如敷料荧光测定所示。本文旨在为在器械相关感染领域工作的科学家提供一种方案，以创建混合物种生物膜，并证明此类生物膜在真实的感染中持续更具毒性。(c)2018 Wiley Periodicals，Inc. J Biomed Mater Res Part B：Appl Biomater，107 B：129-137，2019。
It is becoming increasingly accepted that to understand and model the bacterial colonization and infection of abiotic surfaces requires the use of a biofilm model. There are many bacterial colonizations by at least two primary species, however this is difficult to model in vitro. This study reports the development of a simple mixed-species biofilm model using strains of two clinically significant bacteria: Staphylococcus aureus and Pseudomonas aeruginosa grown on nanoporous polycarbonate membranes on nutrient agar support. Scanning electron microscopy revealed the complex biofilm characteristics of two bacteria blending in extensive extracellular matrices. Using a prototype wound dressing which detects cytolytic virulence factors, the virulence secretion of 30 single and 40 mixed-species biofilms was tested. P. aeruginosa was seen to out-compete S. aureus, resulting in a biofilm with P. aeruginosa dominating. In situ growth of mixed-species biofilm under prototype dressings showed a real-time correlation between the viable biofilm population and their associated virulence factors, as seen by dressing fluorescent assay. This paper aims to provide a protocol for scientists working in the field of device related infection to create mixed-species biofilms and demonstrate that such biofilms are persistently more virulent in real infections. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater, 107B: 129-137, 2019.