The Abscisic Acid Pathway Has Multifaceted Effects on the Accumulation of Bamboo mosaic virus
The Abscisic Acid Pathway Has Multifaceted Effects on the Accumulation of Bamboo mosaic virus
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DOI：
10.1094/mpmi-08-13-0216-r
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发表时间：
2014-02-01
影响因子：
3.5
通讯作者：
Lin, Na-Sheng
中科院分区：
文献类型：
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作者：
Alazem, Mazen;Lin, Kuan-Yu;Lin, Na-Sheng
脱落酸（阿坝）在调节植物对不同生物和非生物胁迫的反应中起着关键作用。然而，阿坝对病毒感染的影响尚未完全了解。在这里，我们描述了阿坝途径对竹花叶病毒（BaMV）和黄瓜花叶病毒（CMV）在两个不同的主机：拟南芥和烟草本塞姆亚纳积累的影响。我们报告说，ABA 2在BaMV和CMV的积累中起着关键作用。ABA 2下游的突变体（aao 3、abi 1 -1、abi 3 -1和abi 4 -1）对BaMV敏感，表明ABA 2下游的阿坝途径对于BaMV抗性是必需的。aba 2 -1突变体降低了BaMV（+）RNA、（-）RNA和外壳蛋白的积累，其中对（-）RNA的影响最显著。这些发现进一步验证了使用病毒诱导的基因沉默和酶联免疫吸附试验在N。本萨米亚纳此外，感染N.本塞姆那与BaMV或CMV的联合处理增加了阿坝含量，激活了SA和阿坝途径，从而破坏了这两个级联反应之间的拮抗作用。我们的研究结果揭示了ABA 2在支持BaMV和CMV积累方面的新作用，与其下游基因的相反作用不同。
Abscisic acid (ABA) plays a key role in modulating plant responses to different biotic and abiotic stresses. However, the effect of ABA on virus infection is not fully understood. Here, we describe the effects of the ABA pathway on the accumulation of Bamboo mosaic virus (BaMV) and Cucumber mosaic virus (CMV) in two different hosts: Arabidopsis thaliana and Nicotiana benthamiana. We report that ABA2 plays a critical role in the accumulation of BaMV and CMV. Mutants downstream of ABA2 (aao3, abi1-1, abi3-1, and abi4-1) were susceptible to BaMV, indicating that the ABA pathway downstream of ABA2 is essential for BaMV resistance. The aba2-1 mutant decreased the accumulation of BaMV (+) RNA, (-) RNA, and coat protein, with the most dramatic effect being observed for (-) RNA. These findings were further validated by the use of virus-induced gene silencing and enzyme-linked immunosorbent assay in N. benthamiana. In addition, infecting N. benthamiana with BaMV or CMV increased ABA contents and activated the SA and ABA pathways, thereby disrupting the antagonism between these two cascades. Our findings uncover a novel role for ABA2 in supporting BaMV and CMV accumulation, distinct from the opposing role of its downstream genes.