microRNA-based cancer cell reprogramming technology (Review)
microRNA-based cancer cell reprogramming technology (Review)
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DOI：
10.3892/etm.2012.558
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发表时间：
2012-07-01
影响因子：
2.7
通讯作者：
Mori, Masaki
中科院分区：
文献类型：
--
作者：
Nishikawa, Shimpei;Ishii, Hideshi;Mori, Masaki
表观遗传修饰在癌症的发生和发展中起着至关重要的作用。通过将确定的生物因子如Oct4、Sox2、Klf4和cMyc引入小鼠和人成纤维细胞中可以实现完全重编程。这些转录因子的引入导致恶性表型行为的改变。最近的研究表明，人类和小鼠的体细胞可以使用microRNA的强制表达重新编程为诱导多能干细胞，这完全消除了异位蛋白表达的需要。考虑到RNA分子的有用性，基于microRNA的重编程技术可能在再生和癌症医学中有未来的应用。
Epigenetic modifications play crucial roles in cancer initiation and development. Complete reprogramming can be achieved through the introduction of defined biological factors such as Oct4, Sox2, Klf4, and cMyc into mouse and human fibroblasts. Introduction of these transcription factors resulted in the modification of malignant phenotype behavior. Recent studies have shown that human and mouse somatic cells can be reprogrammed to become induced pluripotent stem cells using forced expression of microRNAs, which completely eliminates the need for ectopic protein expression. Considering the usefulness of RNA molecules, microRNA-based reprogramming technology may have future applications in regenerative and cancer medicine.